Hirose Mina, Aung Meiji Soe, Fukuda Atsushi, Yahata Shoko, Fujita Yusuke, Saitoh Masato, Hirose Yukito, Urushibara Noriko, Kobayashi Nobumichi
Division of Pediatric Dentistry, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu 061-0293, Japan.
Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan.
Antibiotics (Basel). 2021 Oct 29;10(11):1316. doi: 10.3390/antibiotics10111316.
The acquisition of drug resistance and virulence by staphylococcal species colonizing humans is a growing public health concern. The present study was conducted to investigate the prevalence, antimicrobial resistance and genetic characteristics of isolates from the oral cavity and skin (hand) of systemically healthy subjects with dental disease and dental staff in northern Japan. Among a total of 133 subjects (91 patients and 42 staff), 87 coagulase-positive (83 /4 ) and 162 coagulase-negative (CoNS) isolates were recovered from 59 (44.4%) and 95 (71.4%) subjects, respectively. Three oral isolates were methicillin-resistant (MRSA) (3.6%, 3/83) that were genotyped as ST8-SCC-IVl, ST4775(CC1)-SCC-IVa and ST6562(CC8)-SCC-IVa. Remarkably, the ST6562 isolate harbored PVL genes on ΦSa2usa and type I ACME (arginine catabolic mobile element). Four methicillin-susceptible isolates were identified as belonging to ST1223 and ST2250, which harbored enterotoxin genes and , respectively. Among the fourteen CoNS species identified, methicillin-resistant (MR) isolates were detected in five species (11 isolates, 13.3% of CoNS), with and being the most common. ACME was prevalent in only and . These findings indicated the potential distribution of USA300 clone-like MRSA, toxigenic and MR-CoNS in the oral cavity of dental patients.
定殖于人类的葡萄球菌属获得耐药性和毒力是一个日益严重的公共卫生问题。本研究旨在调查日本北部患有牙病的全身健康受试者和牙科工作人员口腔及皮肤（手部）分离株的流行情况、抗菌药物耐药性和基因特征。在总共133名受试者（91名患者和42名工作人员）中，分别从59名（44.4%）和95名（71.4%）受试者中分离出87株凝固酶阳性（83/4）和162株凝固酶阴性（CoNS）分离株。3株口腔分离株为耐甲氧西林金黄色葡萄球菌（MRSA）（3.6%，3/83），基因分型为ST8-SCC-IVl、ST4775(CC1)-SCC-IVa和ST6562(CC8)-SCC-IVa。值得注意的是，ST6562分离株在ΦSa2usa和I型ACME（精氨酸分解代谢移动元件）上携带PVL基因。4株甲氧西林敏感分离株被鉴定为分别属于ST1223和ST2250，它们分别携带肠毒素基因和。在鉴定出的14种CoNS菌种中，在5种菌种中检测到耐甲氧西林（MR）分离株（11株，占CoNS的13.3%），其中和最为常见。ACME仅在和中普遍存在。这些发现表明，USA300克隆样MRSA、产毒和MR-CoNS在牙科患者口腔中具有潜在分布。
