Kaushik Priyanka, Rawat Kamla, Aswal V K, Kohlbrecher J, Bohidar H B
School of Physical Sciences, Jawaharlal Nehru University, New Delhi, India.
Department of Chemistry, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India.
Carbohydr Polym. 2019 Nov 15;224:115150. doi: 10.1016/j.carbpol.2019.115150. Epub 2019 Jul 31.
Herein, the complex coacervation between in situ formed spherical fluorescent zein nanoparticles and polyanion agar as function of mixing ratio (R=[Agar]/[Zein]) was investigated. This interaction yielded two distinguishable regions (at pH 5.4): Region I (R < 0.2), where fully charge neutralized soluble complexes with protein denaturation was noticed, and Region II (R > 0.2), where overcharged complexes were formed, with R = 0.2 defining the optimum binding. Small angle neutron scattering studies demonstrated that in the low-q region, nanoparticles formed the crosslink junctions and in the persistence regime of high-q region, the data captured the cross-sectional radius ( = 3.5 nm) for agar-zein complexes. The coacervates became more viscoelastic in salt-free samples because both the low frequency storage modulus and crosslink density were found to decrease with mixing ratio. Systematic decrease in storage modulus with ionic strength (0-0.01 M) implied screened Coulomb interaction was responsible for the observed coacervation. Further, we seek to find universality in complex coacervation of zein nanoparticle with biopolymers, and polysaccharides in particular.
在此，研究了原位形成的球形荧光玉米醇溶蛋白纳米颗粒与聚阴离子琼脂之间的复合凝聚作用与混合比（R = [琼脂]/[玉米醇溶蛋白]）的关系。这种相互作用产生了两个可区分的区域（在pH 5.4时）：区域I（R < 0.2），在此区域观察到蛋白质变性的完全电荷中和的可溶性复合物；区域II（R > 0.2），在此区域形成了电荷过量的复合物，R = 0.2定义了最佳结合。小角中子散射研究表明，在低q区域，纳米颗粒形成了交联连接，在高q区域的持久区域，数据捕获了琼脂 - 玉米醇溶蛋白复合物的横截面半径（ = 3.5 nm）。在无盐样品中，凝聚层变得更具粘弹性，因为发现低频储能模量和交联密度均随混合比降低。储能模量随离子强度（0 - 0.01 M）的系统性降低表明，屏蔽的库仑相互作用是观察到的复合凝聚的原因。此外，我们试图在玉米醇溶蛋白纳米颗粒与生物聚合物，特别是多糖的复合凝聚中找到普遍性。
