College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China.
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China.
Food Chem. 2020 Jan 15;303:125394. doi: 10.1016/j.foodchem.2019.125394. Epub 2019 Aug 23.
A simple and effective vesicle based ultrasonic-assisted extraction (UAE) method was developed for extraction of active compounds in functional food. The target analytes were determined by ultra-high performance liquid chromatography with ultraviolet detector. Surfactant vesicle was adopted as extraction solvent. Different operating conditions including the type and concentration of vesicle, extraction time and solid to liquid ratio were investigated by single-factor experiments and response surface methodology. Optimized experimental conditions were 1% (w/v) of DTAB/SDS vesicle, 20 min of extraction time and 160 mg/mL of solid to liquid ratio. The proposed method provided good linearity in the linear range of 10-1000 μg/mL with regression coefficients larger than 0.999, low limits of detection of 27.64-55.67 ng/mL, good precision with relative standard deviations below 0.35%, and satisfactory recoveries of 83.84-90.92% for tested saponins. Consequently, the proposed vesicle based UAE method was well suited for the extraction of saponins in Panax notoginseng.
建立了一种简单有效的基于囊泡的超声辅助提取（UAE）方法，用于从功能性食品中提取活性化合物。目标分析物通过带有紫外检测器的超高效液相色谱法进行测定。采用表面活性剂囊泡作为提取溶剂。通过单因素实验和响应面法研究了不同的操作条件，包括囊泡的类型和浓度、提取时间和固液比。优化的实验条件为 1%（w/v）的 DTAB/SDS 囊泡、20 min 的提取时间和 160 mg/mL 的固液比。该方法在 10-1000μg/mL 的线性范围内具有良好的线性，相关系数大于 0.999，检测限低至 27.64-55.67ng/mL，精密度好，相对标准偏差低于 0.35%，用于测试皂苷的回收率为 83.84-90.92%。因此，所提出的基于囊泡的 UAE 方法非常适合提取三七中的皂苷。
