Programa de Pós-Graduação em Biotecnologia do RENORBIO, Ponto Focal Maranhão, Universidade Federal do Maranhão, São Luís, Brazil.
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
Parasitol Res. 2019 Nov;118(11):3185-3189. doi: 10.1007/s00436-019-06442-3. Epub 2019 Aug 31.
A total of 482 bats representing 32 species and two families were captured in the Amazon forests of the Amapá state in northern Brazil. Nineteen Artibeus planirostris bats (3.9 %) were infested with 160 ticks, all identified as Ornithodoros hasei. Three pools of larvae were screened for rickettsial DNA via polymerase chain reaction (PCR) targeting three rickettsial genes: gltA, ompA and htrA. Only one of them yielded an amplicons of the expected size for all three molecular assays. Comparisons of the obtained sequences including a phylogenetic analysis confirmed the occurrence of "Candidatus Rickettsia wissemanii" in Brazil.
在巴西北部的阿马帕州亚马逊森林中,共捕获了 482 只蝙蝠,代表 32 种和两个科。19 只 A. planirostris 蝙蝠(3.9%)被 160 只蜱虫寄生,所有蜱虫均被鉴定为 O. hasei。通过聚合酶链反应(PCR)针对三个立克次体基因(gltA、ompA 和 htrA)对三个幼虫池进行了立克次体 DNA 的筛查。只有一个幼虫池在所有三个分子检测中都产生了预期大小的扩增子。获得的序列比较包括系统发育分析证实了“Candidatus Rickettsia wissemanii”在巴西的存在。