Guidi Patrizia, Lyons Brett P, Frenzilli Giada
Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
CEFAS Laboratory, Weymouth, Dorset, UK.
Methods Mol Biol. 2019;2031:275-286. doi: 10.1007/978-1-4939-9646-9_14.
Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows for the detection of DNA single- and double-strand breaks, as well as the presence of alkali labile sites and cross-links. Here we describe protocols for the single-cell gel electrophoresis (Comet assay) in its alkaline (pH > 13), mild alkaline (pH = 12.1) and neutral (pH = 8) versions when applied in marine animals.
彗星试验是一种用于评估单个细胞中DNA损伤的快速且通用的技术。它能够检测DNA单链和双链断裂，以及碱不稳定位点和交联的存在。在此，我们描述了将单细胞凝胶电泳（彗星试验）的碱性（pH > 13）、轻度碱性（pH = 12.1）和中性（pH = 8）版本应用于海洋动物时的实验方案。
