海洋动物中的彗星试验。

The Comet Assay in Marine Animals.

作者信息

Guidi Patrizia, Lyons Brett P, Frenzilli Giada

机构信息

Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.

CEFAS Laboratory, Weymouth, Dorset, UK.

出版信息

Methods Mol Biol. 2019;2031:275-286. doi: 10.1007/978-1-4939-9646-9_14.

DOI:10.1007/978-1-4939-9646-9_14

PMID:31473965

Abstract

Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows for the detection of DNA single- and double-strand breaks, as well as the presence of alkali labile sites and cross-links. Here we describe protocols for the single-cell gel electrophoresis (Comet assay) in its alkaline (pH > 13), mild alkaline (pH = 12.1) and neutral (pH = 8) versions when applied in marine animals.

摘要

彗星试验是一种用于评估单个细胞中DNA损伤的快速且通用的技术。它能够检测DNA单链和双链断裂，以及碱不稳定位点和交联的存在。在此，我们描述了将单细胞凝胶电泳（彗星试验）的碱性（pH > 13）、轻度碱性（pH = 12.1）和中性（pH = 8）版本应用于海洋动物时的实验方案。