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增强. 对滴滴涕的生物降解作用。

Enhance the DDT Biodegradation by .

机构信息

Department of Chemistry, Faculty of Science, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia.

Department of Aquaculture, Faculty of Fisheries and Marine Science, Borneo Tarakan University, Amal Lama No. 01, Tarakan Timur, Tarakan, 77123, Indonesia.

出版信息

J Microbiol Biotechnol. 2019 Sep 28;29(9):1424-1433. doi: 10.4014/jmb.1906.06030.

Abstract

DDT is a hydrophobic organic pollutant, which can be bio-accumulated in nature and have adverse consequences on the physical condition of humans and animals. This study investigated the relationship between the white-rot fungus and biosurfactantproducing bacterium associated with the degradation of DDT. The effects of on fungal development were examined using in vitro confrontation assay on a potato dextrose agar (PDA) medium. culture was added to the culture at 1, 3, 5, 7, and 10 ml (1 ml ≈ 1.44 × 10 CFU). After 7 d incubation, about 43% of the initial DDT (12.5 μM) was degraded by the culture only. The augmentation of 7 ml of culture revealed a more highly optimized synergism with DDT degradation being approximately 78% and the ratio of optimization 1.06. According to the confrontational assay, promoted the growth of towards the bacterial colony, with no direct contact between the bacterial cells and mycelium (0.71 cm/day). DDD (1,1-dichloro-2,2-bis(4- chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1- chloro-2,2-bis(4-chlorophenyl) ethylene) were identified as metabolic products, indicating that the could enhance the DDT biodegradation by .

摘要

滴滴涕是一种疏水性有机污染物,它可以在自然界中生物累积,并对人类和动物的身体状况产生不利影响。本研究调查了与滴滴涕降解相关的白腐真菌和生物表面活性剂产生细菌之间的关系。通过在马铃薯葡萄糖琼脂(PDA)培养基上进行体外对抗试验,研究了 对真菌发育的影响。在 培养物中分别添加 1、3、5、7 和 10 ml(1 ml ≈ 1.44 × 10 CFU)的 培养物。7 d 孵育后,仅 培养物就降解了约 43%的初始滴滴涕(12.5 μM)。添加 7 ml 的 培养物显示出与滴滴涕降解更高度的协同作用,降解率约为 78%,优化比为 1.06。根据对抗性试验, 促进了 向细菌菌落的生长,细菌细胞和菌丝体之间没有直接接触(0.71 cm/day)。鉴定出代谢产物为 DDD(1,1-二氯-2,2-双(4-氯苯基)乙烷)、DDE(1,1-二氯-2,2-双(4-氯苯基)乙烯)和 DDMU(1-氯-2,2-双(4-氯苯基)乙烯),表明 可以增强 对滴滴涕的生物降解作用。

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