Nabilah Badzlin, Purnomo Adi Setyo, Rizqi Hamdan Dwi, Putro Herdayanto Sulistyo, Nawfa Refdinal
Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya, 60111, Indonesia.
Heliyon. 2022 Feb 16;8(2):e08963. doi: 10.1016/j.heliyon.2022.e08963. eCollection 2022 Feb.
Methylene blue (MB) is one of synthetic dyes that is used in the textile industry which is difficult to degrade in nature. Previously, the brown-rot fungus (BRF) had shown a good ability to degrade MB, however, the decolorization ability was relatively still low and had a long period of incubation. Therefore, improvement of process is needed to increase the ability of to decolorize MB. In this study, the effect of bacterium addition on MB biodecolorization by the BRF in potato dextrose broth (PDB) medium was investigated. The amount of that was added to the culture of were 2, 4, 6, 8, and 10 mL (1 mL ≈ 1.39 × 10 CFU). The cultures had ability to decolorize MB (100 mg/L) at 30 °C after 7 days incubation. The highest percentage of MB biodecolorization was obtained at addition of 10 mL of approximately 89%, while biodecolorization process by particularly was approximately 17%. The MB degradation metabolites by mixed cultures of and 10 mL of were Azure A, thionine, glucose-MB, CHNSO and CHNO. This study indicated that the addition of could enhance MB biodecolorization by fungus . Besides that, this study also indicated that mixed cultures of and has great potential for high efficiency, fast and cheap dye wastewater treatment.
亚甲基蓝（MB）是纺织工业中使用的合成染料之一，在自然环境中难以降解。此前，褐腐菌（BRF）已显示出良好的降解MB的能力，然而，其脱色能力相对仍然较低，且培养周期较长。因此，需要改进工艺以提高其对MB的脱色能力。在本研究中，研究了添加细菌对BRF在马铃薯葡萄糖肉汤（PDB）培养基中对MB进行生物脱色的影响。添加到BRF培养物中的细菌量分别为2、4、6、8和10 mL（1 mL≈1.39×10 CFU）。培养物在30℃下培养7天后能够对MB（100 mg/L）进行脱色。在添加10 mL细菌时获得了最高的MB生物脱色率，约为89%，而仅BRF的脱色率约为17%。BRF与10 mL细菌混合培养对MB的降解代谢产物为天青A、硫堇、葡萄糖-MB、CHNSO和CHNO。本研究表明，添加细菌可以增强BRF对MB的生物脱色能力。除此之外，本研究还表明，BRF与细菌的混合培养在高效、快速且廉价地处理染料废水方面具有巨大潜力。
