Pflug Irving J, Melgaard Hans L, Schaffer Shawn M, Lysfjord Jack P
Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108, USA.
PDA J Pharm Sci Technol. 2008 Mar-Apr;62(2):85-96.
This is the report of a project carried out to determine the microbial-kill characteristics of saturated steam plus hydrogen peroxide (H2O2) using a specially-constructed test apparatus. Spores on stainless-steel planchets were inserted into a flowing gaseous atmosphere of steam plus H2O2 for a timed exposure to the lethal agent. The specially-designed test apparatus and its operating parameters are described. Geobacillus stearothermophilus (former name, Bacillus stearothermophilus) spore-death rates were evaluated in several spore-planchet handling modes. Enumeration microbial recovery methods were used. The data were analyzed using survivor-curve methods; D-values were calculated using the initial number of spores per planchet and the number of spores surviving the process. Extensive tests were carried out using Geobacillus stearothermophilus spores; limited tests were carried out using Bacillus smithii ATCC 51232 (former name, Bacillus coagulans), Bacillus macerans, and Bacillus subtilis, subtilis ATCC 35021 spores (former name, Bacillus subtilis, CCC 5230, Kerns 15U). For G. stearothermophilus spores subjected to steam plus H2O2 and recovered using the 2B procedure (planchets deposited in sterile, 100-mL bottles containing 50.0 mL of buffer immediately after they were subjected to the steam-H2O2 condition; 11 experiments), the mean D-value was 0.48 min at 2,500 ppm and 0.22 min at 7,500 ppm. The application of steam plus H2O2 to the sterilization of barrier isolator enclosures is discussed.
这是一份关于使用特制测试设备来测定饱和蒸汽加过氧化氢（H₂O₂）杀菌特性的项目报告。将不锈钢载片上的孢子置于蒸汽加H₂O₂的流动气体氛围中，进行定时暴露于致死剂的处理。文中描述了该特制测试设备及其运行参数。在几种孢子载片处理模式下评估了嗜热栖热放线菌（曾用名，嗜热脂肪芽孢杆菌）的孢子死亡率。采用了微生物计数回收方法。使用存活曲线法对数据进行分析；D值是根据每个载片上的初始孢子数和处理后存活的孢子数计算得出的。使用嗜热栖热放线菌孢子进行了大量测试；使用史密斯芽孢杆菌ATCC 51232（曾用名，凝结芽孢杆菌）、浸麻芽孢杆菌和枯草芽孢杆菌ATCC 35021（曾用名，枯草芽孢杆菌CCC 5230、Kerns 15U）的孢子进行了有限测试。对于经过蒸汽加H₂O₂处理并采用2B程序回收的嗜热栖热放线菌孢子（载片在经过蒸汽 - H₂O₂处理后立即放入含有50.0 mL缓冲液的无菌100 mL瓶中；11次实验），在2500 ppm时平均D值为0.48分钟，在7500 ppm时为0.22分钟。文中还讨论了蒸汽加H₂O₂在隔离器屏障灭菌中的应用。
