Department of Pathology, Xuzhou Health Hospital of Women and Children, Xuzhou, P.R. China.
Int J Biol Markers. 2019 Sep;34(3):284-291. doi: 10.1177/1724600819872765. Epub 2019 Sep 3.
Aberrant expression of circular RNAs (circRNAs) is implicated in tumorigenesis and disease progression. However, the underlying molecular mechanisms and physiological functions of hsa_circ_0005075 in human colorectal cancer are still poorly understood.
Quantitative real-time PCR (qRT-PCR) was performed to detect the expression level of hsa_circ_0005075 in colorectal cancer. Correlations of hsa_circ_0005075 expression with pathological parameters and overall survival were assessed. CCK-8 and Transwell invasion assays were utilized to determine the effect of hsa_circ_0005075 on proliferation and invasion of colorectal cancer cells.
Hsa_circ_0005075 was overexpressed in colorectal cancer tissues compared with paracancerous tissues and intestinal polyps. Its expression level was associated with distal metastasis, invasion, tumor node metastasis stage, and tumor diameter in colorectal cancer, and was negatively correlated with overall survival of patients with colorectal cancer. Moreover, enforced expression of hsa_circ_0005075 potentiated the proliferation and invasive behavior of colorectal cancer cells.
Our findings suggested that hsa_circ_0005075 expression was increased in colorectal cancer and might serve as a promising diagnostic mark and therapy target of colorectal cancer.
环状 RNA（circRNAs）的异常表达与肿瘤发生和疾病进展有关。然而，hsa_circ_0005075 在人结直肠癌中的潜在分子机制和生理功能仍知之甚少。
采用实时定量 PCR（qRT-PCR）检测结直肠癌中 hsa_circ_0005075 的表达水平。评估 hsa_circ_0005075 表达与病理参数和总生存期的相关性。CCK-8 和 Transwell 侵袭实验用于确定 hsa_circ_0005075 对结直肠癌细胞增殖和侵袭的影响。
与癌旁组织和肠息肉相比，hsa_circ_0005075 在结直肠癌组织中表达上调。其表达水平与结直肠癌的远端转移、侵袭、肿瘤淋巴结转移分期和肿瘤直径有关，与结直肠癌患者的总生存期呈负相关。此外，hsa_circ_0005075 的过表达增强了结直肠癌细胞的增殖和侵袭行为。
我们的研究结果表明，hsa_circ_0005075 在结直肠癌中表达增加，可能作为结直肠癌有前途的诊断标志物和治疗靶点。
