Hwang Jung Eun, Hwang Sun-Goo, Jung In Jung, Han Sung Min, Ahn Joon-Woo, Kim Jin-Baek
National Institute of Ecology, Research Center for Endangered Species, Division of Restoration Research, Yeongyang, Republic of Korea.
Kangwon Natl University, Department of Applied Plant Sciences, Plant Genomics Lab, Chuncheon, Republic of Korea.
Genet Mol Biol. 2020 Mar 2;43(1):e20180273. doi: 10.1590/1678-4685-GMB-2018-0273. eCollection 2020.
Ionizing radiation has a substantial effect on physiological and biochemical processes in plants via induction of transcriptional changes and diverse genetic alterations. Previous microarray analysis showed that rice OsFBX322, which encodes a rice F-box protein, was downregulated in response to three types of ionizing radiation: gamma irradiation, ion beams, and cosmic rays. In order to characterize the radiation-responsive genes in rice, OsFBX322 was selected for further analysis. OsFBX322 expression patterns in response to radiation were confirmed using quantitative RT-PCR. Transient expression of a GFP-OsFBX322 fusion protein in tobacco leaf epidermis indicated that OsFBX322 is localized to the nucleus. To determine the effect of OsFBX322 expression on radiation response, OsFBX322 was overexpressed in Arabidopsis. Transgenic overexpression lines were more sensitive to gamma irradiation than control plants. These results suggest that OsFBX322 plays a negative role in the defense response to radiation in plants. In addition, we obtained four co-expression genes of OsFBX322 by specific co-expression networks using the ARANCE. Quantitative RT-PCR showed that the four genes were also downregulated after exposure to the three types of radiation. These results imply that the co-expressed genes may serve as key regulators in the radiation response pathway in plants.
电离辐射通过诱导转录变化和多种基因改变，对植物的生理和生化过程产生重大影响。先前的微阵列分析表明，编码水稻F-box蛋白的水稻OsFBX322在受到三种电离辐射（γ射线照射、离子束和宇宙射线）时表达下调。为了表征水稻中的辐射响应基因，选择OsFBX322进行进一步分析。使用定量RT-PCR确认了OsFBX322对辐射的响应表达模式。GFP-OsFBX322融合蛋白在烟草叶表皮中的瞬时表达表明OsFBX322定位于细胞核。为了确定OsFBX322表达对辐射响应的影响，在拟南芥中过表达了OsFBX322。转基因过表达株系对γ射线照射比对照植物更敏感。这些结果表明，OsFBX322在植物对辐射的防御反应中起负作用。此外，我们使用ARANCE通过特定的共表达网络获得了OsFBX322的四个共表达基因。定量RT-PCR表明，这四个基因在暴露于三种辐射后也下调。这些结果表明，共表达基因可能是植物辐射响应途径中的关键调节因子。
