Suppr超能文献

脱水素OpsDHN1的核定位由富含组氨酸的基序决定。

Nuclear localization of the dehydrin OpsDHN1 is determined by histidine-rich motif.

作者信息

Hernández-Sánchez Itzell E, Maruri-López Israel, Ferrando Alejandro, Carbonell Juan, Graether Steffen P, Jiménez-Bremont Juan F

机构信息

Laboratorio de Biología Molecular de Hongos y Plantas, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica AC San Luis Potosí, México.

Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas Valencia, Spain.

出版信息

Front Plant Sci. 2015 Sep 7;6:702. doi: 10.3389/fpls.2015.00702. eCollection 2015.

Abstract

The cactus OpsDHN1 dehydrin belongs to a large family of disordered and highly hydrophilic proteins known as Late Embryogenesis Abundant (LEA) proteins, which accumulate during the late stages of embryogenesis and in response to abiotic stresses. Herein, we present the in vivo OpsDHN1 subcellular localization by N-terminal GFP translational fusion; our results revealed a cytoplasmic and nuclear localization of the GFP::OpsDHN1 protein in Nicotiana benthamiana epidermal cells. In addition, dimer assembly of OpsDHN1 in planta using a Bimolecular Fluorescence Complementation (BiFC) approach was demonstrated. In order to understand the in vivo role of the histidine-rich motif, the OpsDHN1-ΔHis version was produced and assayed for its subcellular localization and dimer capability by GFP fusion and BiFC assays, respectively. We found that deletion of the OpsDHN1 histidine-rich motif restricted its localization to cytoplasm, but did not affect dimer formation. In addition, the deletion of the S-segment in the OpsDHN1 protein affected its nuclear localization. Our data suggest that the deletion of histidine-rich motif and S-segment show similar effects, preventing OpsDHN1 from getting into the nucleus. Based on these results, the histidine-rich motif is proposed as a targeting element for OpsDHN1 nuclear localization.

摘要

仙人掌OpsDHN1脱水蛋白属于一大类无序且高度亲水的蛋白质家族,即晚期胚胎发生丰富(LEA)蛋白,这类蛋白在胚胎发生后期以及对非生物胁迫作出响应时积累。在此,我们通过N端GFP翻译融合展示了OpsDHN1在体内的亚细胞定位;我们的结果揭示了GFP::OpsDHN1蛋白在本氏烟草表皮细胞中的细胞质和细胞核定位。此外,还通过双分子荧光互补(BiFC)方法证明了OpsDHN1在植物中的二聚体组装。为了了解富含组氨酸基序在体内的作用,制备了OpsDHN1-ΔHis变体,并分别通过GFP融合和BiFC试验检测其亚细胞定位和二聚体能力。我们发现,删除OpsDHN1富含组氨酸的基序会将其定位限制在细胞质中,但不影响二聚体形成。此外,OpsDHN1蛋白中S片段的缺失影响其细胞核定位。我们的数据表明,富含组氨酸基序和S片段的缺失显示出相似的效果,阻止OpsDHN1进入细胞核。基于这些结果,富含组氨酸的基序被认为是OpsDHN1细胞核定位的靶向元件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f5a/4561349/4942542852ac/fpls-06-00702-g0001.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验