Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin, 17104, South Korea.
Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, South Korea.
Plant Cell Rep. 2022 May;41(5):1229-1242. doi: 10.1007/s00299-022-02852-3. Epub 2022 Mar 6.
To further understand the regulatory mechanism for anther dehiscence in rice, we carried out transcriptome analysis for the following two tissues: the anther wall and pollen at the anthesis stage. With the anatomical meta-expression data, in addition to these tissues, the differentially expressed genes (DEGs) between the two tissues were further refined to identify 1,717 pollen-preferred genes and 534 anther wall-preferred genes. A GUS transgenic line and RT-qPCR analysis for anther wall-preferred genes supported the fidelity of our gene candidates for further analysis. The refined DEGs were functionally classified through Gene Ontology (GO) enrichment and MapMan analyses. Through the analysis of cis-acting elements and alternative splicing variants, we also suggest the feature of regulatory sequences in promoter regions for anther wall-preferred expression and provide information of the unique splicing variants in anther wall. Subsequently, it was found that hormone signaling and the resulting transcriptional regulation pathways may play an important role in anther dehiscence and anther wall development. Our results could provide useful insights into future research to broaden the molecular mechanism of anther dehiscence or anther wall development in rice.
为了进一步了解水稻花粉囊开裂的调控机制，我们对以下两个组织进行了转录组分析：花药壁和开花期的花粉。通过解剖元表达数据，除了这些组织之外，我们还进一步细化了两个组织之间的差异表达基因（DEGs），以鉴定 1717 个花粉偏好基因和 534 个花药壁偏好基因。一个 GUS 转基因系和花药壁偏好基因的 RT-qPCR 分析支持了我们对进一步分析的候选基因的准确性。通过基因本体论（GO）富集和 MapMan 分析对精细的 DEGs 进行了功能分类。通过顺式作用元件和可变剪接变体的分析，我们还提出了花药壁偏好表达的启动子区调控序列的特征，并提供了花药壁中独特剪接变体的信息。随后发现，激素信号及其导致的转录调控途径可能在花粉囊开裂和花药壁发育中起重要作用。我们的研究结果为未来研究提供了有用的见解，以扩大水稻花粉囊开裂或花药壁发育的分子机制。
