Xia Xiaoting, Qu Kaixing, Li Fangyu, Jia Peng, Chen Qiuming, Chen Ningbo, Zhang Jicai, Chen Hong, Huang Bizhi, Lei Chuzhao
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
Yunnan Academy of Grassland and Animal Science, Kunming 650212, Yunnan, China.
Animals (Basel). 2019 Sep 2;9(9):641. doi: 10.3390/ani9090641.
Yunling cattle are a composite beef cattle breed, combining Brahman (1/2), Murray Grey (1/4) and Local Yunnan cattle (1/4), that was developed in Yunnan, China in the 1980s. Understanding the genetic information of Yunling cattle is of great significance to the development of reasonable breeding strategies for this breed. In the present study, we assessed the current genetic status of Yunling cattle in Yunnan Province (China) by analyzing the variability of the whole mitochondrial genome of 129 individuals. Altogether, 129 sequences displayed 47 different haplotypes. The haplotype diversity and the average number of nucleotide differences were 0.964 and 128.074, respectively. Phylogenetic analyses classified Yunling cattle into seven haplogroups: T1, T2, T3, T4, T6, I1 and I2. Haplogroup I1 was found to be predominant (41.86%), followed by T3 (28.68%). Furthermore, we also identified a novel haplogroup, T6, and defined the sub-haplogroup I1a in Yunling cattle. According to the formation process of Yunling cattle (local Yunnan cattle as the maternal line), the high genetic diversities in the mitochondria of Yunling cattle could be due to the complex maternal origin of local Yunnan cattle. Further studies about local Yunnan breeds are necessary to determine the exact source of haplogroup T6 in Yunling cattle. Our results will be useful for the evaluation and effective management of Yunling cattle.
云岭牛是一种杂交肉牛品种，由婆罗门牛（1/2）、墨累灰牛（1/4）和云南本地牛（1/4）杂交而成，于20世纪80年代在中国云南培育而成。了解云岭牛的遗传信息对于制定该品种合理的育种策略具有重要意义。在本研究中，我们通过分析129头个体的线粒体全基因组变异性，评估了中国云南省云岭牛的当前遗传状况。总共，129个序列显示出47种不同的单倍型。单倍型多样性和平均核苷酸差异数分别为0.964和128.074。系统发育分析将云岭牛分为七个单倍群：T1、T2、T3、T4、T6、I1和I2。发现单倍群I占主导地位（41.86%），其次是T3（28.68%）。此外，我们还鉴定出一个新的单倍群T6，并在云岭牛中定义了亚单倍群I1a。根据云岭牛的形成过程（以云南本地牛为母系），云岭牛线粒体的高遗传多样性可能归因于云南本地牛复杂的母系起源。有必要对云南本地品种进行进一步研究，以确定云岭牛中T6单倍群的确切来源。我们的结果将有助于云岭牛的评估和有效管理。
