Oshima Masao, Taniguchi Yojiro, Akasaka Maiko, Abe Kiyomi, Ichikawa Hiroaki, Tabei Yutaka, Tanaka Junichi
Institute of Agrobiological Sciences, NARO, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Institute of Crop Science, NARO, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.
Breed Sci. 2019 Jun;69(2):244-254. doi: 10.1270/jsbbs.18151. Epub 2019 Mar 15.
To overcome a limitation to the breeding of autogamous crops, recurrent selection using transgenic male sterility (RSUTMS) has been proposed. In this system, negatively or positively selectable marker traits are required along with dominant transgenic male sterility. Anthocyanin pigmentation is an excellent marker trait. Two regulatory genes for MYB and bHLH and a structural gene for DFR are required for anthocyanin pigmentation in rice. Therefore, to apply anthocyanin pigmentation as a marker trait in various rice genotypes, coordinated expression of the three genes is required. In this study, we developed a leaf sheath-specific promoter and introduced three genes- and , driven by the promoter, and /, driven by the leaf sheath-specific promoter-into the rice genome. Leaf sheath-specific pigmentation was confirmed in all seven genotypes tested, which included and cultivars. Analysis of genome sequence data from 25 cultivars showed that the strategy of conferring leaf sheath-specific anthocyanin pigmentation by introduction of these three genes would be effective for a wide range of genotypes and will be applicable to RSUTMS.
为克服自花授粉作物育种的一个限制因素，人们提出了利用转基因雄性不育进行轮回选择（RSUTMS）的方法。在该系统中，除了显性转基因雄性不育外，还需要负向或正向选择标记性状。花青素色素沉着是一种优良的标记性状。水稻中花青素色素沉着需要两个MYB和bHLH调控基因以及一个DFR结构基因。因此，为了将花青素色素沉着作为各种水稻基因型的标记性状，需要这三个基因的协调表达。在本研究中，我们开发了一个叶鞘特异性启动子，并将由该启动子驱动的三个基因以及由叶鞘特异性启动子驱动的/，导入水稻基因组。在所测试的所有七个基因型中均证实了叶鞘特异性色素沉着，其中包括和品种。对25个品种的基因组序列数据进行分析表明，通过导入这三个基因赋予叶鞘特异性花青素色素沉着的策略对广泛的基因型有效，并将适用于RSUTMS。
