Department of Animal Sciences, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC, USA.
Department of Energy and Environmental Systems, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC, USA.
Mediators Inflamm. 2019 Aug 1;2019:7420468. doi: 10.1155/2019/7420468. eCollection 2019.
Exposure to hog barn organic dust contributes to occupational lung diseases, which are mediated by inflammatory and oxidative stress pathways. Isoprostanes-a family of eicosanoids produced by oxidation of phospholipids by oxygen radicals-are biomarkers of pulmonary oxidative stress. Importantly, 8-isoprostane has been implicated as a key biomarker and mediator of oxidative stress because it is a potent pulmonary vasoconstrictor. Antioxidants found in fruits and vegetables hold promise for preventing or reducing effects of oxidative stress-related diseases including chronic bronchitis and chronic obstructive pulmonary disease (COPD). Here, we investigated 8-isoP and oxidant production by organic dust-exposed airway epithelial cells and the inhibitory effects of an extract from calyces of the sorrel plant, , on oxidant-producing pathways. Confluent cultures of normal human tracheobronchial epithelial cells were pretreated or not with 1% sorrel extract prior to 5% dust extract (DE) exposure. Following DE treatments, live cells, cell-free supernatants, or cell extracts were evaluated for the presence of 8-isoprostane, superoxide, hydrogen peroxide, nitric oxide, hydroxyl radical, peroxynitrite, and catalase activity to evaluate sorrel's inhibitory effect on oxidative stress. The well-known radical scavenging antioxidant, N-acetyl cysteine (NAC), was used for comparisons with sorrel. DE exposure augmented the production of all radicals measured including 8-isoprostane ( value < 0.001), which could be inhibited by NAC or sorrel. Among reactive oxygen and nitrogen species generated in response to DE exposure, sorrel had no effect on HO production and NAC had no significant effect on NO production. The observations reported here suggest a possible role for sorrel in preventing 8-isoprostane and oxidant-mediated stress responses in bronchial epithelial cells exposed to hog barn dust. These findings suggest a potential role for oxidative stress pathways in mediating occupational lung diseases and antioxidants within sorrel and NAC in reducing dust-mediated oxidative stress within the airways of exposed workers.
猪舍有机粉尘暴露会导致职业性肺部疾病，这些疾病是通过炎症和氧化应激途径介导的。异前列烷是一类由氧自由基氧化磷脂产生的类花生酸，是肺氧化应激的生物标志物。重要的是，8-异前列烷已被认为是氧化应激的关键生物标志物和介质，因为它是一种有效的肺血管收缩剂。水果和蔬菜中的抗氧化剂有望预防或减少与氧化应激相关的疾病的影响，包括慢性支气管炎和慢性阻塞性肺疾病（COPD）。在这里，我们研究了有机粉尘暴露的气道上皮细胞中 8-异前列烷和氧化剂的产生，以及菠菜根提取物对氧化剂产生途径的抑制作用。用 1%菠菜根提取物预处理或不预处理正常人气道上皮细胞的汇合培养物，然后用 5%粉尘提取物（DE）暴露。在 DE 处理后，评估活细胞、无细胞上清液或细胞提取物中 8-异前列烷、超氧阴离子、过氧化氢、一氧化氮、羟基自由基、过氧亚硝酸盐和过氧化氢酶活性的存在，以评估菠菜根提取物对氧化应激的抑制作用。使用众所周知的自由基清除抗氧化剂 N-乙酰半胱氨酸（NAC）与菠菜根提取物进行比较。DE 暴露增加了所有测量自由基的产生，包括 8-异前列烷（ 值<0.001），这可以被 NAC 或菠菜根提取物抑制。在 DE 暴露引起的活性氧和氮物种中，菠菜根提取物对 HO 的产生没有影响，NAC 对 NO 的产生也没有显著影响。这里报告的观察结果表明，菠菜根提取物可能在预防猪舍粉尘暴露的支气管上皮细胞中 8-异前列烷和氧化剂介导的应激反应中发挥作用。这些发现表明，氧化应激途径可能在介导职业性肺部疾病中起作用，菠菜根提取物和 NAC 中的抗氧化剂可能在减少暴露工人气道中粉尘介导的氧化应激方面发挥作用。
