Liu Dandan, Cheng Lili, Tang Lili, Yang Lie, Jiang Zhongjuan, Song Xixia, Kang Qinghua, Yao Dandan, Chen Si, Ru Jiarong, Zhang Lili, Wu Guangwen, Yuan Hongmei
Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150086, China.
Int J Mol Sci. 2025 Jun 3;26(11):5360. doi: 10.3390/ijms26115360.
Hyperhydricity is a frequently occurring physiological disorder in plant tissue culture that impairs the regeneration and survival of vitrified plant materials, leading to significant losses and complicating research applications. Although numerous studies have focused on strategies to mitigate hyperhydricity, its molecular mechanisms remain poorly characterized. In this study, transcriptome sequencing and endogenous hormone content analysis were conducted on hyperhydric and normal callus of flax ( L.). Transcriptomic analysis revealed 2698 differentially expressed genes (DEGs) between these two tissue types. Pathway analysis through KEGG demonstrated that these DEGs predominantly linked to metabolic processes including phenylpropanoid formation, MAPK signaling cascades, and plant hormone signal transduction. Moreover, quantification of endogenous hormone levels indicated a significant reduction in all hormones except cytokinins (ZRs) in hyperhydric tissues. The observed disruption in endogenous hormone levels suggests its potential role in the development of plant vitrification. These findings provide valuable insights into the molecular processes underlying hyperhydricity, contributing to a more comprehensive understanding of this phenomenon.
玻璃化是植物组织培养中经常出现的一种生理紊乱现象，它会损害玻璃化植物材料的再生和存活能力，导致重大损失，并使研究应用复杂化。尽管众多研究聚焦于减轻玻璃化的策略，但其分子机制仍未得到充分表征。在本研究中，对亚麻（L.）的玻璃化愈伤组织和正常愈伤组织进行了转录组测序和内源激素含量分析。转录组分析揭示了这两种组织类型之间有2698个差异表达基因（DEG）。通过KEGG进行的通路分析表明，这些DEG主要与包括苯丙烷类物质形成、MAPK信号级联和植物激素信号转导在内的代谢过程相关。此外，内源激素水平的定量分析表明，玻璃化组织中除细胞分裂素（ZRs）外的所有激素含量均显著降低。内源激素水平的这种变化表明其在植物玻璃化发育中可能发挥的作用。这些发现为玻璃化现象背后的分子过程提供了有价值的见解，有助于更全面地理解这一现象。
