Low- and intermediate-copy-number cloning vectors based on the Pseudomonas plasmid pVS1.
作者信息
Itoh Y, Soldati L, Leisinger T, Haas D
机构信息
Dept. of Bacteriology, Shinshu University School of Medicine, Matsumoto, Japan.
出版信息
Antonie Van Leeuwenhoek. 1988;54(6):567-73. doi: 10.1007/BF00588392.
PMID:3148293
Abstract
The cloning vector pME290 (6.8 kb), which is derived from the Pseudomonas plasmid pVS1 and has about 7 copies, was mutagenized in vitro to provide derivatives with altered copy numbers. Thus, pME292 (about 1-3 copies) and pME294 (about 15-20 copies) were isolated. These vectors were used in the characterization of the P. aeruginosa argF gene encoding ornithine carbamoyltransferase.
摘要
Zotero 插件