Al-Hamdani M M, Atkinson M E, Mayhew T M
Cell Tissue Res. 1979 Sep 1;200(3):495-509. doi: 10.1007/BF00234859.
Changes in the volumes and surfaces of subcellular compartments of unstimulated small lymphocytes and immunoblasts in mouse axillary lymph nodes have been established using stereological techniques. Blast transformation was induced in vivo with dinitrochlorobenzene (DNCB). Cell samples were obtained by random sampling regimes applied at light and electron microscopic levels. From electron micrographs the volume densities of euchromatin, heterochromatin, nucleoli, mitochondria, Golgi apparatus and rough endoplasmic reticulum were determined. Cell surface/volume ratios were also computed. By estimating mean nuclear volumes using light microscopy, it was possible to calculate absolute compartmental volumes and to evaluate the plasma membrane surface areas of average cells. Transformation in this model was characterized by a considerable cellular hypertrophy and a substantial increase in plasmalemma surface. Hypertrophy was the consequence of increases in the volumes of all measured intracellular compartments, notably euchromatin and "residual cytoplasm" (including ground cytoplasm and free ribosomes). These changes are discussed in the context of the altered metabolic status of cells.
利用体视学技术，已确定了小鼠腋窝淋巴结中未受刺激的小淋巴细胞和免疫母细胞亚细胞区室的体积和表面积变化。用二硝基氯苯（DNCB）在体内诱导细胞发生胚细胞转化。通过在光学和电子显微镜水平应用随机抽样方法获取细胞样本。从电子显微照片中测定常染色质、异染色质、核仁、线粒体、高尔基体和粗面内质网的体积密度。还计算了细胞表面/体积比。通过光学显微镜估计平均核体积，得以计算绝对区室体积并评估平均细胞的质膜表面积。该模型中的转化特征为细胞显著肥大以及质膜表面积大幅增加。肥大是所有测量的细胞内区室体积增加的结果，尤其是常染色质和“残余细胞质”（包括基础细胞质和游离核糖体）。结合细胞代谢状态的改变对这些变化进行了讨论。
