Tan Zhongming, Qi Xian, Gu Ling, Bao Changjun, Tang Fenyang, Zhu Yefei
Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health, Jiangsu Provincial Centre for Disease Control and Prevention, Nanjing, Jiangsu Province, China.
Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health, Jiangsu Provincial Centre for Disease Control and Prevention, Nanjing, Jiangsu Province, China
Am J Trop Med Hyg. 2014 Sep;91(3):574-6. doi: 10.4269/ajtmh.13-0633. Epub 2014 Jul 7.
An outbreak of anthrax was reported in Lianyungang, Jiangsu Province, China in 2012. Laboratory confirmation of cases was made by detection of Bacillus anthracis genes rpoB, pagA, and cap using real-time polymerase chain reaction (PCR); source tracking was conducted by multiple locus variable-number tandem-repeat analysis (MLVA) and pagA sequencing using DNA extracted from case specimens and meat from a suspected slaughtered cow. The genotypes were MLVA type 57 and pagA genotype I. Combined with the field epidemiological data, the four cutaneous anthrax cases most likely were caused by butchering of the sick cow. Backward tracing of animal cases identified the region of origin, and some public health measures, such as reactive or preventative animal vaccination for cattle, intersectoral cooperation, ensuring proper pre-slaughter inspection, and educating butchers and villagers about this disease, could be used to prevent B. anthracis infection.
2012年，中国江苏省连云港市报告了一起炭疽疫情。通过实时聚合酶链反应（PCR）检测炭疽杆菌基因rpoB、pagA和cap对病例进行实验室确诊；利用从病例标本和疑似屠宰奶牛的肉中提取的DNA，通过多位点可变数目串联重复分析（MLVA）和pagA测序进行溯源。基因型为MLVA 57型和pagA基因型I。结合现场流行病学数据，这4例皮肤炭疽病例很可能是由于宰杀病牛所致。对动物病例进行反向追踪确定了起源地区，一些公共卫生措施，如对牛进行反应性或预防性动物疫苗接种、部门间合作、确保适当的宰前检查以及对屠夫和村民进行该病防治知识教育等，可用于预防炭疽杆菌感染。
