College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.
Academic Unit of Ophthalmology, Bristol Medical School, University of Bristol, Bristol, BS8 1TD, UK.
Sci Rep. 2019 Sep 4;9(1):12735. doi: 10.1038/s41598-019-48893-9.
It is well documented that four gene families, including the glycerophosphate acyltransferases (GPATs), acylglycerophosphate acyltransferases (AGPATs), lipid phosphate phosphohydrolases (LPINs) and diacylglycerol acyltransferases (DGATs), are involved in the glycerophosphate pathway of de novo triglyceride (TG) biosynthesis in mammals. However, no systematic analysis has been conducted to characterize the gene families in poultry. In this study, the sequences of gene family members in the glycerophosphate pathway were obtained by screening the public databases. The phylogenetic tree, gene structures and conserved motifs of the corresponding proteins were evaluated. Dynamic expression changes of the genes at different developmental stages were analyzed by qRT-PCR. The regulatory characteristics of the genes were analyzed by in vivo experiments. The results showed that the GPAT, AGPAT and LPIN gene families have 2, 7 and 2 members, respectively, and they were classified into 2, 4 and 2 cluster respectively based on phylogenetic analysis. All of the genes except AGPAT1 were extensively expressed in various tissues. Estrogen induction upregulated the expression of GPAM and AGPAT2, downregulated the expression of AGPAT3, AGPAT9, LPIN1 and LPIN2, and had no effect on the expression of the other genes. These findings provide a valuable resource for further investigation of lipid metabolism in liver of chicken.
有大量文献记载，包括甘油磷酸酰基转移酶（GPATs）、酰基甘油磷酸酰基转移酶（AGPATs）、脂磷酸磷酸水解酶（LPINs）和二酰基甘油酰基转移酶（DGATs）在内的四个基因家族参与了哺乳动物甘油磷酸途径的从头甘油三酯（TG）生物合成。然而，目前还没有系统的分析来描述家禽中的基因家族。在本研究中，通过筛选公共数据库获得了甘油磷酸途径中基因家族成员的序列。评估了相应蛋白的系统发育树、基因结构和保守基序。通过 qRT-PCR 分析了不同发育阶段基因的动态表达变化。通过体内实验分析了基因的调控特征。结果表明，GPAT、AGPAT 和 LPIN 基因家族分别有 2、7 和 2 个成员，根据系统发育分析分别分为 2、4 和 2 个簇。除了 AGPAT1 之外，所有基因在各种组织中都广泛表达。雌激素诱导上调了 GPAM 和 AGPAT2 的表达，下调了 AGPAT3、AGPAT9、LPIN1 和 LPIN2 的表达，而对其他基因的表达没有影响。这些发现为进一步研究鸡肝脏中的脂质代谢提供了有价值的资源。
