Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, China.
School of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, 467044, China.
Sci Rep. 2017 Apr 25;7(1):1159. doi: 10.1038/s41598-017-01357-4.
The mitogen-activated protein kinase (MAPK) cascade, which is a major signal transduction pathway widely distributed in eukaryotes, has an important function in plant development and stress responses. However, less information is known regarding the MAPKKK and MAPKK gene families in the important fruit crop banana. In this study, 10 MAPKK and 77 MAPKKK genes were identified in the banana genome, and were classified into 4 and 3 subfamilies respectively based on phylogenetic analysis. Majority of MAPKKK and MAPKK genes in the same subfamily shared similar gene structures and conserved motifs. The comprehensive transcriptome analysis indicated that MAPKKK-MAPKK genes is involved in tissue development, fruit development and ripening, and response to abiotic stress of drought, cold and salt in two banana genotypes. Interaction networks and co-expression assays demonstrated that MAPK signaling cascade mediated network participates in multiple stress signaling, which was strongly activated in Fen Jiao (FJ). The findings of this study advance understanding of the intricately transcriptional control of MAPKKK-MAPKK genes and provide robust candidate genes for further genetic improvement of banana.
丝裂原活化蛋白激酶（MAPK）级联反应是一种广泛存在于真核生物中的主要信号转导途径，在植物发育和应激反应中具有重要功能。然而，关于重要水果作物香蕉中的 MAPKKK 和 MAPKK 基因家族的信息知之甚少。在本研究中，在香蕉基因组中鉴定了 10 个 MAPKK 和 77 个 MAPKKK 基因，并根据系统发育分析将其分别归类为 4 个和 3 个亚家族。同一亚家族中的大多数 MAPKKK 和 MAPKK 基因具有相似的基因结构和保守基序。综合转录组分析表明，MAPKKK-MAPKK 基因参与了组织发育、果实发育和成熟以及对干旱、寒冷和盐胁迫的反应。相互作用网络和共表达分析表明，MAPK 信号级联介导的网络参与了多种胁迫信号转导，在 Fen Jiao（FJ）中强烈激活。本研究的结果加深了对 MAPKKK-MAPKK 基因转录调控的理解，并为进一步遗传改良香蕉提供了强有力的候选基因。
