Guo Pan-Pan, Jin Xin, Zhang Jun-Fang, Li Qiang, Yan Chang-Guo, Li Xiang-Zi
Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, China.
Healthcare International Innovation Institute, Jiangmen 529020, China.
Animals (Basel). 2023 Mar 29;13(7):1195. doi: 10.3390/ani13071195.
Triacylglycerols (TAGs) are a major component of intramuscular fat. Diacylglycerol O-acyltransferase 2() expression determines the rate of TAG synthesis. The purpose of this study was to investigate the role of in the differentiation of Yanbian cattle preadipocytes and lipid metabolism-related signalling pathways. Bovine preadipocytes were infected with overexpression and interfering adenovirus vectors of . The effects on the differentiation of Yanbian cattle preadipocytes were examined using molecular and transcriptomic techniques, including differentially expressed genes (DEGs) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis. overexpression significantly increased ( < 0.05) intracellular TAG, adiponectin, and lipid droplet (LD) contents. Moreover, it upregulated ( < 0.05) peroxisome proliferator-activated receptor γ , CCAAT/enhancer binding protein , and fatty acid binding protein 4 mRNA expression. In contrast, knockdown reduced intracellular TAG and LD content and downregulated ( < 0.05) , mannosyl (alpha-1,3-)-glycoproteinbeta-1,2-N-acetylglucosaminyltransferase, lipin 1,1-acylglycerol-3-phosphate O-acyltransferase 4, and acetyl-CoA carboxylase alpha mRNA expression. Between DGAT2-overexpressing preadipocytes and normal cells, 208 DEGs were identified, including 106 upregulated and 102 downregulated genes. KEGG pathway analysis revealed DEGs mainly enriched in PPAR signalling and AMP-activated protein kinase pathways, cholesterol metabolism, and fatty acid biosynthesis. These results demonstrated that regulated preadipocyte differentiation and LD and TAG accumulation by mediating the expression of adipose differentiation-, lipid metabolism-, and fatty acid synthesis-related genes.
三酰甘油（TAGs）是肌内脂肪的主要成分。二酰甘油O-酰基转移酶2（DGAT2）的表达决定了TAG的合成速率。本研究的目的是探讨DGAT2在延边牛前体脂肪细胞分化及脂质代谢相关信号通路中的作用。用DGAT2的过表达和干扰腺病毒载体感染牛前体脂肪细胞。使用分子和转录组学技术，包括差异表达基因（DEGs）和京都基因与基因组百科全书（KEGG）通路分析，检测其对延边牛前体脂肪细胞分化的影响。DGAT2过表达显著增加（P<0.05）细胞内TAG、脂联素和脂滴（LD）含量。此外，它上调（P<0.05）过氧化物酶体增殖物激活受体γ、CCAAT/增强子结合蛋白α和脂肪酸结合蛋白4的mRNA表达。相反，DGAT2敲低降低了细胞内TAG和LD含量，并下调（P<0.05）甘露糖基（α-1,3-）-糖蛋白β-1,2-N-乙酰葡糖胺基转移酶、脂素1、1-酰基甘油-3-磷酸O-酰基转移酶4和乙酰辅酶A羧化酶α的mRNA表达。在过表达DGAT2的前体脂肪细胞和正常细胞之间，鉴定出208个差异表达基因，包括106个上调基因和102个下调基因。KEGG通路分析显示差异表达基因主要富集于PPAR信号通路和AMP激活的蛋白激酶通路、胆固醇代谢和脂肪酸生物合成。这些结果表明，DGAT2通过介导脂肪分化、脂质代谢和脂肪酸合成相关基因的表达来调节前体脂肪细胞分化以及LD和TAG的积累。
