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尿液雌激素作为大熊猫（Ailuropoda melanoleuca）有活力妊娠的非侵入性生物标志物。

Urinary estrogens as a non-invasive biomarker of viable pregnancy in the giant panda (Ailuropoda melanoleuca).

机构信息

MRC Centre for Reproductive Health, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.

Laboratory of Chemical Analysis, Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, 9820, Merelbeke, Belgium.

出版信息

Sci Rep. 2019 Sep 4;9(1):12772. doi: 10.1038/s41598-019-49288-6.

DOI:10.1038/s41598-019-49288-6
PMID:31484972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6726647/
Abstract

Female giant pandas show complex reproductive traits, being seasonally monoestrus, displaying a variable length embryonic diapause and exhibiting pseudopregnancy. Currently, there is no confirmatory non-invasive biomarker of blastocyst implantation or pregnancy. This study aimed to monitor urinary estrogens across gestation in pregnancy (n = 4), pseudopregnancy (n = 4) and non-birth cycles (n = 5) in the giant panda. A pregnancy-specific profile of estrogens corrected for urinary specific gravity was identified during the gestation period. Pregnant females showed increasing concentrations of estrogens for 29 days until birth, no increase was observed during pseudopregnancy and the two profiles were distinguishable from each other for the final 2 weeks of the cycle suggesting the estrogens are of placental origin. This allowed a nomogram, starting at a known fixed point during the cycle, to be created and tested with cycles of known outcome, and cycles which were inseminated but did not result in a birth. Non-birth profiles showed deviations from that of pregnancy. We believe these deviations indicate the point of failure of the placenta to support a developing cub. Non-invasive longitudinal monitoring of estrogen concentrations therefore has the potential to be developed as a panda pregnancy test to predict viable cub development.

摘要

雌性大熊猫表现出复杂的生殖特征，即季节性单发情，具有可变长度的胚胎休眠，并表现出假孕。目前，尚无确认胚胎着床或妊娠的非侵入性生物标志物。本研究旨在监测大熊猫妊娠（n=4）、假孕（n=4）和非分娩周期（n=5）期间的尿液雌激素。在妊娠期间，确定了经尿液比重校正的妊娠特异性雌激素特征。怀孕的雌性大熊猫的雌激素浓度在 29 天内持续增加，直到分娩，而在假孕期间没有增加，这两种特征在周期的最后两周可以区分开来，表明雌激素来源于胎盘。这允许创建一个从周期中已知固定点开始的图表，并使用已知结果的周期以及已经受精但未导致分娩的周期进行测试。非分娩周期特征与妊娠特征不同。我们认为这些偏差表明胎盘支持发育幼仔的能力失败。因此，非侵入性的雌激素浓度纵向监测有可能作为大熊猫妊娠试验来预测可育幼仔的发育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa39/6726647/8d59b15b1e16/41598_2019_49288_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa39/6726647/7a2eeacc5f5f/41598_2019_49288_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa39/6726647/2f03eef843fe/41598_2019_49288_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa39/6726647/9f9e347538e9/41598_2019_49288_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa39/6726647/8d59b15b1e16/41598_2019_49288_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa39/6726647/7a2eeacc5f5f/41598_2019_49288_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa39/6726647/2f03eef843fe/41598_2019_49288_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa39/6726647/9f9e347538e9/41598_2019_49288_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa39/6726647/8d59b15b1e16/41598_2019_49288_Fig4_HTML.jpg

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