Arokiyaraj S, Varghese R, Ali Ahmed B, Duraipandiyan V, Al-Dhabi N A
Department of Food Science and Technology, Sejong University, Republic of Korea.
Department of Biotechnology, PRIST University, Thanjavur, Tamil Nadu 613401, India.
Saudi J Biol Sci. 2019 Feb;26(2):378-381. doi: 10.1016/j.sjbs.2018.10.011. Epub 2018 Oct 16.
Keratinase degrading Bacillus was isolated from the halophilic environment in Tamilnadu, India and keratinase production was optimized using wheat bran substrate. Of the screened bacterial isolates, four were found to have the ability to produce keratinolytic enzyme. The process parameters were optimized using one-variable-at-a-time approach and response surface methodology. Supplementation of 1% lactose supported more keratinase production (120 U/g). Among the selected nitrogen sources, addition of casein significantly enhanced maximum keratinase production (132.5 U/g). Among the ions, manganese chloride significantly enhanced keratinsase production (102.6 U/g), however addition of zinc sulphate and copper sulphate decreased keratinase production. The maximum keratinase production was obtained in the wheat bran medium containing 1% lactose, 0.5% manganese with 80% moisture (292 U/g). Statistics based contour plots were generated to explore the variations in the response surface and to find the relationship between the keratinase yield and the bioprocess conditions.
从印度泰米尔纳德邦的嗜盐环境中分离出了降解角蛋白酶的芽孢杆菌，并使用麦麸底物优化了角蛋白酶的生产。在筛选出的细菌分离物中，发现有四种具有产生角蛋白分解酶的能力。采用一次一个变量的方法和响应面法对工艺参数进行了优化。添加1%的乳糖可促进更多角蛋白酶的产生（120 U/g）。在所选的氮源中，添加酪蛋白显著提高了角蛋白酶的最大产量（132.5 U/g）。在离子中，氯化锰显著提高了角蛋白酶的产量（102.6 U/g），然而添加硫酸锌和硫酸铜则降低了角蛋白酶的产量。在含有1%乳糖、0.5%锰且水分含量为80%的麦麸培养基中获得了最大角蛋白酶产量（292 U/g）。生成了基于统计的等高线图，以探索响应面的变化，并找出角蛋白酶产量与生物工艺条件之间的关系。
