Liu Fang, Wei Wei, Fang Jianhua, Wu Yuanyuan, Zhang Qifei, Wang Cunxin, Ye Lihong, Liu Min
Hangzhou Red Cross Hospital, Hangzhou 310003, China.
Zhejiang Chinese Medical University, Hangzhou 310053, China.
Saudi J Biol Sci. 2019 Feb;26(2):390-394. doi: 10.1016/j.sjbs.2018.11.011. Epub 2018 Nov 20.
To explore how the use of a laser needle-knife affects vertebroarterial morphology and protein expression of PI-3K, AKT and VEGF in the carotid artery of a rabbit model of cervical spondylotic arteriopathy (CSA), and to determine its primary treatment mechanism.
The CSA rabbit model consisted of 30 rabbits with CSA that were randomly divided into a model group (n = 10), an acupuncture group (n = 10) and a laser needle-knife group (n = 10) plus a further 10 wild type rabbits placed into a normal group. Rabbits in the acupuncture group were treated by needling the Fengchi (GB 20) and Jiaji (EX-B2) acupoints, while those in the laser needle-knife group were treated with a laser needle-knife on the Jiaji (EX-B2) near the spinous process of the fifth cervical vertebra, the rabbit in both groups immobilized during treatment. Rabbits in the other two groups received no treatment, but were immobilized for a similar duration. The morphology of the right vertebral artery and the distribution of pore size before and after treatment were compared using BET and SEM, and the protein expression of PI-3K, AKT and VEGF in the carotid artery of the four groups measured using Western blot analysis.
The pore diameter and specific surface area of the right vertebral artery increased after treatment, as did the number of micropores. Compared with the normal group, the protein expression of PI-3K, AKT and VEGF in the carotid artery of the model group was significantly lower (P < 0.05), while that of the laser needle-knife group was significantly higher (P < 0.05 and P < 0.01). In addition, rabbits from the acupuncture and laser needle-knife groups demonstrated significantly higher levels of protein expression of PI-3K and VEGF in the carotid artery compared with the model group (P < 0.05 and P < 0.01).
By promoting micrangium hyperplasia within the vertebral artery of rabbits with CSA, treatment with a laser needle-knife modified the protein expression of PI-3K, AKT and VEGF, suggesting that laser needle-knife therapy possibly treats CSA though these signaling pathways.
探讨激光针刀对颈椎病性动脉病（CSA）兔模型颈动脉椎动脉形态及PI-3K、AKT和VEGF蛋白表达的影响，并确定其主要治疗机制。
将30只CSA兔模型随机分为模型组（n = 10）、针刺组（n = 10）和激光针刀组（n = 10），另取10只野生型兔作为正常组。针刺组兔针刺风池（GB 20）和夹脊（EX - B2）穴位，激光针刀组兔于第五颈椎棘突旁夹脊（EX - B2）处用激光针刀治疗，两组治疗时均将兔固定。另外两组兔不治疗，但固定时间相似。采用BET和SEM比较治疗前后右侧椎动脉形态及孔径分布，采用蛋白质免疫印迹法检测四组兔颈动脉中PI-3K、AKT和VEGF的蛋白表达。
治疗后右侧椎动脉孔径和比表面积增加，微孔数量也增加。与正常组相比，模型组兔颈动脉中PI-3K、AKT和VEGF的蛋白表达显著降低（P < 0.05），而激光针刀组显著升高（P < 0.05和P < 0.01）。此外，针刺组和激光针刀组兔颈动脉中PI-3K和VEGF的蛋白表达水平与模型组相比显著升高（P < 0.05和P < 0.01）。
激光针刀治疗通过促进CSA兔椎动脉内微血管增生，改变PI-3K、AKT和VEGF的蛋白表达，提示激光针刀疗法可能通过这些信号通路治疗CSA。
