Poluri Raghavendra Tejo Karthik, Beauparlant Charles Joly, Droit Arnaud, Audet-Walsh Étienne
Department of Molecular Medicine, Faculty of Medicine, Université Laval, Axe Endocrinologie - Néphrologie du Centre de recherche du CHU de Québec - Université Laval, Québec City, Québec, Canada.
Centre de recherche sur le cancer de l'Université Laval, Québec City, Québec, Canada.
Data Brief. 2019 Aug 9;25:104372. doi: 10.1016/j.dib.2019.104372. eCollection 2019 Aug.
Prostate cancer (PCa) is the most frequent cancer in North American men and PCa cells rely on the androgen receptor (AR) for growth and survival. To understand the effect of AR in cancer cells, we have treated LNCaP and LAPC4 cells, two immortalized human PCa cells , with the synthetic androgen R1881 and then performed RNA-seq analyses. High quality sequencing data have been analyzed using our bioinformatic pipeline which consists of FastQC for quality controls, Trimmomatic for trimming, and Kallisto for pseudoalignment to the transcriptome. Differentially expressed genes were identified using DESeq2 after adjustment for false-discovery rate (FDR values < 0.05) and Relative Log Expression (RLE) normalization. Gene Set Enrichment Analysis (GSEA) was also performed to identify biological pathways significantly modulated by androgens. GSEA analyses identified the androgen signaling pathway, as well as several metabolic pathways, as significantly enriched following androgen stimulation. These analyses highlight the most significant metabolic pathways up-regulated following AR activation. Raw and processed RNA-seq data were deposited and made publicly available on the Gene Expression Omnibus (GEO; GSE128749). These data have been incorporated in a recent article describing the functions of AR as a master regulator of PCa cell metabolism. For more details about interpretation of these results, please refer to "Functional genomics studies reveal the androgen receptor as a master regulator of cellular energy metabolism in prostate cancer" by Gonthier et al. (doi: 10.1016/j.jsbmb.2019.04.016).
前列腺癌（PCa）是北美男性中最常见的癌症，前列腺癌细胞的生长和存活依赖于雄激素受体（AR）。为了了解AR在癌细胞中的作用，我们用合成雄激素R1881处理了两种永生化的人前列腺癌细胞LNCaP和LAPC4，然后进行了RNA测序分析。使用我们的生物信息学流程对高质量测序数据进行了分析，该流程包括用于质量控制的FastQC、用于修剪的Trimmomatic以及用于与转录组进行伪比对的Kallisto。在调整错误发现率（FDR值<0.05）和相对对数表达（RLE）标准化后，使用DESeq2鉴定差异表达基因。还进行了基因集富集分析（GSEA），以确定受雄激素显著调节的生物学途径。GSEA分析确定雄激素信号通路以及几种代谢途径在雄激素刺激后显著富集。这些分析突出了AR激活后上调最显著的代谢途径。原始和处理后的RNA测序数据已存放在基因表达综合数据库（GEO；GSE128749）中并公开提供。这些数据已被纳入最近一篇描述AR作为前列腺癌细胞代谢主要调节因子功能的文章中。有关这些结果解释的更多详细信息，请参考Gonthier等人的“功能基因组学研究揭示雄激素受体是前列腺癌细胞能量代谢的主要调节因子”（doi：10.1016/j.jsbmb.2019.04.016）。
