College of Agriculture, Yanbian University, Yanji, China.
Chungbuk National University, Cheongju, Korea.
Anim Sci J. 2019 Nov;90(11):1417-1425. doi: 10.1111/asj.13280. Epub 2019 Sep 4.
Kaempferol (KAE) is a natural flavonoid present in different plant species and exhibits anti-inflammatory, antioxidant, and anticancer therapeutic properties. In the present study, we investigated the influence and underlying mechanisms of KAE supplementation on porcine oocytes during in vitro aging. The results show that KAE treatment can alleviate the aging-related reduction of developmental competence. We observed that the blastocyst production rate in aged oocytes treated with 0.1 μM KAE was significantly higher than in untreated aging oocytes (36.78 ± 0.86% vs. 27.55 ± 2.60%, respectively, p < .05). The KAE-treated aging oocytes had significantly reduced levels of reactive oxygen species (p < .05). Furthermore, the mRNA levels of the embryonic pluripotency-related genes Oct4, NANOG, and ITGA5 were significantly increased in blastocysts derived from KAE-treated oocytes (p < .05). During excessive oocyte culture, KAE treatment maintained the mitochondrial membrane potential and reduced apoptosis; however, this was not observed in untreated aging oocytes. In conclusion, our results suggest that KAE treatment can alleviate the aging of porcine oocytes by reducing oxidative stress and improving mitochondrial function.
山柰酚（KAE）是一种存在于不同植物物种中的天然类黄酮，具有抗炎、抗氧化和抗癌治疗特性。在本研究中，我们研究了 KAE 补充剂对体外老化猪卵母细胞的影响及其潜在机制。结果表明，KAE 处理可以减轻与老化相关的发育能力下降。我们观察到，用 0.1μM KAE 处理的老化卵母细胞的囊胚产率明显高于未处理的老化卵母细胞（分别为 36.78±0.86%和 27.55±2.60%，p<0.05）。KAE 处理的老化卵母细胞中活性氧（ROS）水平明显降低（p<0.05）。此外，来自 KAE 处理卵母细胞的囊胚中胚胎多能性相关基因 Oct4、NANOG 和 ITGA5 的 mRNA 水平显著增加（p<0.05）。在过度培养卵母细胞期间，KAE 处理维持线粒体膜电位并减少凋亡；然而，在未处理的老化卵母细胞中未观察到这种情况。总之，我们的结果表明，KAE 处理可以通过减少氧化应激和改善线粒体功能来减轻猪卵母细胞的老化。
