Vasanthkumar Thriveni, Hanumanthappa Manjunatha, Lakshminarayana Rangaswamy
Department of Post-graduation Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Jnana Sahyadri, Shankaraghatta, Shivamogga, Karnataka, 577 451, India.
Department of Biotechnology, Acharya Institute of Technology, Jnana Sahyadri, Shankaraghatta, Bangalore, Karnataka, 560 107, India.
Cytotechnology. 2019 Oct;71(5):963-976. doi: 10.1007/s10616-019-00338-x. Epub 2019 Sep 5.
The mechanism of action of treatment of either curcumin or capsaicin or in combination on LPS (Lipopolysaccharide) induced inflammatory gene expression in peripheral blood mononuclear cells (PBMCs) was investigated using RT-PCR and in silico docking methods. RT-PCR analysis has shown that the curcumin and capsaicin significantly reduced LPS induced over expression of COX-2, IL-6 and TGF-β in PBMCs. Whereas combined molecules demonstrated synergistic response on the reduction of COX-2, IL-6 and TGF-β over expression in LPS induced PBMCs as compared to individual molecules. Further, The docking of curcumin and capsaicin at the active pockets of COX-2, IL-6 and TGF-β has shown - 3.90, - 4.49 and - 5.61 kcal/mol binding energy for curcumin and - 3.80, - 4.78 and - 5.76 kcal/mol binding energy for capsaicin, while multiple ligand simultaneous docking (MLSD) of both molecules has shown higher binding energy of - 4.24, - 5.35 and - 5.83 kcal/mol respectively. This has demonstrated the efficacy of combined curcumin and capsaicin against the LPS induced expression of pro-inflammatory cytokines in PBMCs. These results attributed the coordinated positive modulation on biochemical and molecular cellular process by combined curcumin and capsaicin as compared to individual molecules.
采用逆转录聚合酶链反应（RT-PCR）和计算机对接方法，研究了姜黄素、辣椒素单独或联合使用对脂多糖（LPS）诱导的外周血单个核细胞（PBMCs）炎症基因表达的作用机制。RT-PCR分析表明，姜黄素和辣椒素显著降低了LPS诱导的PBMCs中COX-2、IL-6和TGF-β的过表达。与单个分子相比，联合分子对LPS诱导的PBMCs中COX-2、IL-6和TGF-β过表达的降低表现出协同反应。此外，姜黄素和辣椒素在COX-2、IL-6和TGF-β活性口袋处的对接显示，姜黄素的结合能分别为-3.90、-4.49和-5.61 kcal/mol，辣椒素的结合能分别为-3.80、-4.78和-5.76 kcal/mol，而两种分子的多配体同时对接（MLSD）显示结合能分别更高，为-4.24、-5.35和-5.83 kcal/mol。这证明了姜黄素和辣椒素联合使用对LPS诱导的PBMCs中促炎细胞因子表达的有效性。这些结果表明，与单个分子相比，姜黄素和辣椒素联合使用对生化和分子细胞过程具有协同正向调节作用。
