Genome Research Chair, Department of Biochemistry, College of Science King Saud University, Riyadh, Kingdom of Saudi Arabia.
College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia.
PLoS One. 2019 Sep 5;14(9):e0220931. doi: 10.1371/journal.pone.0220931. eCollection 2019.
LncRNA Prostate cancer non-coding RNA (PRNCR1) is downregulated in many types of cancer. The current case-control study was performed on 144 patients with colorectal cancer and 130 matching controls. Genotyping was performed using TaqMan assays for four Single Nucleotide Polymorphisms (SNPs) in PRNCR1. RNAsnp Web Server was used to detect variations in the secondary structure for each SNP. The genotyping analysis for SNP rs1456315 showed increased association with colorectal cancer with the homozygous CC variant allele (OR: 2.09; χ2 = 4.95; CI: 1.08-4.02; p = 0.02), the minor allele frequency, and additive genotype, respectively (OR: 1.55; χ2 = 6.24; CI: 1.09-2.19; p = 0.01) & (OR: 1.64; χ2 = 4.04; CI: 1.01-2.67; p = 0.04). A risk association was also observed among younger age patients (≤57) and in female patients as well as in patients with tumors of the colon. For the other SNPs tested (rs16901946, rs13252298, rs1016343), no significant association was observed. The secondary structure of the rs1456315 mutant is different from that of the wild-type. Our findings suggest that the upregulation of PRNCR1 and its variants is associated with increased risk of colorectal cancer in Saudi patients, indicating that PRNCR1 might be a unique and valuable signature for predicting the risk of colorectal cancer in a Saudi population.
LncRNA 前列腺癌非编码 RNA (PRNCR1) 在许多类型的癌症中下调。本病例对照研究在 144 例结直肠癌患者和 130 例匹配对照中进行。使用 TaqMan 测定法对 PRNCR1 中的四个单核苷酸多态性 (SNP) 进行基因分型。使用 RNAsnp Web Server 检测每个 SNP 的二级结构变化。SNP rs1456315 的基因分型分析显示与结直肠癌的关联增加，纯合 CC 变异等位基因 (OR: 2.09; χ2 = 4.95; CI: 1.08-4.02; p = 0.02)、次要等位基因频率和加性基因型，分别为 (OR: 1.55; χ2 = 6.24; CI: 1.09-2.19; p = 0.01) & (OR: 1.64; χ2 = 4.04; CI: 1.01-2.67; p = 0.04)。在年龄较小的患者 (≤57 岁)、女性患者以及结肠肿瘤患者中也观察到风险关联。对于其他测试的 SNP(rs16901946、rs13252298、rs1016343)，未观察到显著关联。rs1456315 突变体的二级结构与野生型不同。我们的研究结果表明，PRNCR1 及其变体的上调与沙特患者结直肠癌风险增加相关，表明 PRNCR1 可能是预测沙特人群结直肠癌风险的独特且有价值的标志物。
