Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China.
Xi'an Yuensun Biological Technology Co., Ltd. Pioneering R&D Park, Xi'an, 710075, China.
Mol Nutr Food Res. 2019 Dec;63(23):e1900521. doi: 10.1002/mnfr.201900521. Epub 2019 Sep 15.
Obesity is associated with gut microbiome dysbiosis. Mannose oligosaccharide (MOS) has been reported to be a potential prebiotic. The present study is aimed to determine the effects of MOS on western-diet-induced obesity and to uncover the mediating roles of the gut microbiota and microbial metabolites.
Three-month-old male ICR mice are fed with a high-fat and high-fructose diet for 8 weeks. The diet-induced obese mice are then orally administrated with MOS (100 and 200 mg kg d ) for 4 weeks. MOS significantly reduces bodyweight gain, insulin resistance, fatty liver, and inflammatory responses in obese mice. MOS also stimulates lipolysis and inhibits lipogenesis in the adipose tissues. Moreover, MOS restructures the gut microbiome by enhancing the abundance of Bifidobacterium and Lactobacillus in obese mice. The microbial metabolite SCFAs are also increased in the feces and serum. Correlation analysis indicates that the appetite suppression and lipid-lowering effects of MOS are highly correlated with the butyrate levels.
MOS suppresses the appetite, which results in less lipid deposition. The lower appetite is likely due to an altered gut microbiome and elevated SCFAs production. MOS may be a potential nutraceutical used in body weight management and gut health improvement.
肥胖与肠道微生物失调有关。甘露寡糖（MOS）已被报道为一种潜在的益生元。本研究旨在确定 MOS 对西式饮食诱导肥胖的影响，并揭示肠道微生物群和微生物代谢物的介导作用。
3 月龄雄性 ICR 小鼠用高脂肪高果糖饮食喂养 8 周。然后，饮食诱导肥胖的小鼠用 MOS（100 和 200mg/kg/d）口服给药 4 周。MOS 可显著减轻肥胖小鼠的体重增加、胰岛素抵抗、脂肪肝和炎症反应。MOS 还可刺激脂肪组织中的脂肪分解和抑制脂肪生成。此外，MOS 通过增加肥胖小鼠中双歧杆菌和乳酸杆菌的丰度来重塑肠道微生物群。粪便和血清中的微生物代谢物 SCFAs 也增加了。相关性分析表明，MOS 的食欲抑制和降血脂作用与丁酸盐水平高度相关。
MOS 抑制食欲，从而减少脂质沉积。较低的食欲可能是由于肠道微生物群的改变和 SCFAs 产量的增加。MOS 可能是一种用于体重管理和改善肠道健康的潜在营养保健品。
