College of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Department of Food Science, Foshan University, Foshan 528000, China.
Mar Drugs. 2019 Sep 4;17(9):520. doi: 10.3390/md17090520.
Ultraviolet (UV) irradiation is a potent inducer for skin photoaging. This paper investigated the anti-photoaging effects of the acetylated and amidated hexapeptide (AAH), originally identified from , in (Ultraviolet B) UVB-irradiated Human immortalized keratinocytes (Hacats) and mice. The results demonstrated that AAH had much lower toxicity on Hacats than the positive matrixyl (81.52% vs. 5.32%). Moreover, AAH reduced MDA content by 49%; increased SOD, CAT, and GSH-Px activities by 103%, 49%, and 116%, respectively; decreased MMP-1 and MMP-3 expressions by 27% and 29%, respectively, compared to UVB-irradiated mice. Employing isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics, 60 differential proteins were identified, and major metabolic pathways were determined. Network analysis indicated that these differential proteins were mapped into an interaction network composed of two core sub-networks. Collectively, AAH is protective against UVB-induced skin photoaging and has potential application in skin care cosmetics.
紫外线（UV）辐射是皮肤光老化的有效诱导因素。本文研究了从 中鉴定出的乙酰化和酰胺化六肽（AAH）对（中长波紫外线 B）UVB 照射的人永生化角质形成细胞（Hacats）和小鼠的抗光老化作用。结果表明，AAH 对 Hacats 的毒性比阳性基质（81.52%对 5.32%）低得多。此外，与 UVB 照射的小鼠相比，AAH 使 MDA 含量降低了 49%；使 SOD、CAT 和 GSH-Px 活性分别增加了 103%、49%和 116%；使 MMP-1 和 MMP-3 的表达分别降低了 27%和 29%。采用同位素标记相对和绝对定量（iTRAQ）基于蛋白质组学的方法，鉴定出 60 个差异蛋白，并确定了主要代谢途径。网络分析表明，这些差异蛋白被映射到一个由两个核心子网组成的相互作用网络中。综上所述，AAH 可预防 UVB 诱导的皮肤光老化，具有在皮肤护理化妆品中应用的潜力。
