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Nat Biotechnol. 2017 Aug 8;35(8):725-731. doi: 10.1038/nbt.3893.
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Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads.单轮循环器:从短读长和长读长测序数据中解析细菌基因组组装结果
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The genome-sequenced variant of Campylobacter jejuni NCTC 11168 and the original clonal clinical isolate differ markedly in colonization, gene expression, and virulence-associated phenotypes.空肠弯曲菌NCTC 11168的基因组测序变体与原始克隆临床分离株在定植、基因表达和毒力相关表型方面存在显著差异。
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确保您的基因组组装具有可重复性和清晰度,以适应未来需求。

Future-Proofing Your Genome Assembly for Reproducibility and Clarity.

作者信息

Baltrus David A, Cuomo Christina A, Dennehy John J, Dunning Hotopp Julie C, Maresca Julia A, Newton Irene L G, Rasko David A, Rokas Antonis, Roux Simon, Stajich Jason E

机构信息

School of Plant Sciences, University of Arizona, Tucson, Arizona, USA

Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

出版信息

Microbiol Resour Announc. 2019 Sep 5;8(36):e00954-19. doi: 10.1128/MRA.00954-19.

DOI:10.1128/MRA.00954-19
PMID:31488541
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6728651/
Abstract

Descriptions of resources, like the genome assemblies reported in , are often frozen at their time of publication, yet they will need to be interpreted in the midst of continually evolving technologies. It is therefore important to ensure that researchers accessing published resources have access to all of the information required to repeat, interpret, and extend these original analyses. Here, we provide a set of suggestions to help make certain that published resources remain useful and repeatable for the foreseeable future.

摘要

对资源的描述,比如[具体文献中]报道的基因组组装结果,往往在发表时就固定下来了,但在技术不断发展的过程中仍需要对其进行解读。因此,确保访问已发表资源的研究人员能够获取重复、解读和扩展这些原始分析所需的所有信息非常重要。在此,我们提供了一系列建议,以确保已发表的资源在可预见的未来仍然有用且可重复。