Department of Chemistry, Anna University, University College of Engineering, Dindigul, Tamilnadu, India.
Department of Chemistry, SSM Institute of Engineering and Technology, Dindigul, Tamilnadu, India.
Luminescence. 2020 Feb;35(1):90-97. doi: 10.1002/bio.3701. Epub 2019 Sep 5.
A phenothiazine-rhodamine (PTRH) fluorescent dyad was synthesized and its ability to selectively sense Zn ions in solution and in in vitro cell lines was tested using various techniques. When compared with other competing metal ions, the PTRH probe showed the high selectivity for Zn ions that was supported by electronic and emission spectral analyses. The emission band at 528 nm for the PTRH probe indicated the ring closed form of PTRH, as for Zn ion binding to PTRH, the λ get shift to 608 nm was accompanied by a pale yellow to pink colour (under visible light) and green to pinkish red fluorescence emission (under UV light) due to ring opening of the spirolactam moiety in the PTRH ligand. Spectral overlap of the donor emission band and the absorption band of the ring opened form of the acceptor moiety contributed towards the fluorescence resonance energy transfer ON mechanism for Zn ion detection. The PTRH sensor had the lowest detection limit for Zn , found to be 2.89 × 10 M. The sensor also demonstrated good sensing application with minimum toxicity for in vitro analyses using HeLa cells.
合成了一种吩噻嗪-罗丹明（PTRH）荧光二联体，并通过各种技术测试了其在溶液中和体外细胞系中选择性检测 Zn 离子的能力。与其他竞争金属离子相比，PTRH 探针对 Zn 离子具有高选择性，这得到了电子和发射光谱分析的支持。PTRH 探针的 528nm 发射带表明 PTRH 处于闭环形式，由于 Zn 离子与 PTRH 结合，λ 发生红移至 608nm 伴随着由于 PTRH 配体中螺环内酰胺部分的开环，可见光是浅黄色到粉红色（），紫外光是绿色到粉红色（）荧光发射（）。供体发射带与受体部分开环形式的吸收带的光谱重叠有助于 Zn 离子检测的荧光共振能量转移 ON 机制。PTRH 传感器对 Zn 的检测限最低，为 2.89×10 。该传感器还展示了良好的传感应用，对 HeLa 细胞的体外分析具有最小的毒性。
