Gowers Sally A N, Hamaoui Karim, Vallant Natalie, Hanna George B, Darzi Ara, Casanova Daniel, Papalois Vassilios, Boutelle Martyn G
Department of Bioengineering , Imperial College London , UK . Email:
Department of Surgery & Cancer , Imperial College London , UK.
Anal Methods. 2018 Nov 28;10(44):5273-5281. doi: 10.1039/c8ay01807c. Epub 2018 Nov 5.
Online organ monitoring could provide clinicians with critical information regarding organ health prior to transplantation and could aid clinical decision-making. This paper presents the methodology of online microdialysis for real-time monitoring of human organs . We describe how rapid sampling microdialysis can be incorporated with organ perfusion machines to create a robust organ monitoring system and demonstrate its use in monitoring human and porcine kidneys as well as human and porcine pancreases. In this paper we also show the potential usefulness of this methodology for evaluating novel interventions in a research setting. The analysis system can be configured either to analyse two analytes in one organ, allowing for ratiometric analysis, or alternatively to monitor one analyte in two organs simultaneously, allowing direct comparison. It was found to be reliable over long monitoring periods in real clinical use. The results clearly show that the analysis system is sensitive to differences between organs and therefore has huge potential as an organ monitoring tool.
在线器官监测可为临床医生在移植前提供有关器官健康的关键信息，并有助于临床决策。本文介绍了用于人体器官实时监测的在线微透析方法。我们描述了如何将快速采样微透析与器官灌注机相结合，以创建一个强大的器官监测系统，并展示其在监测人肾和猪肾以及人胰腺和猪胰腺中的应用。在本文中，我们还展示了该方法在研究环境中评估新型干预措施的潜在用途。该分析系统既可以配置为分析一个器官中的两种分析物，进行比率分析，也可以配置为同时监测两个器官中的一种分析物，进行直接比较。在实际临床使用中，经过长时间监测发现该系统是可靠的。结果清楚地表明，该分析系统对不同器官之间的差异敏感，因此作为一种器官监测工具具有巨大潜力。
