Alessi M C, Juhan-Vague I, Kooistra T, Declerck P J, Collen D
Laboratory of Hematology, CHU Timone, Marseille, France.
Thromb Haemost. 1988 Dec 22;60(3):491-4.
Secretion of plasminogen activator inhibitor 1 (PAI-1) by cultures of human umbilical vein endothelial cells and human hepatocellular cell line Hep G2 was evaluated after insulin stimulation. The secretion of PAI-1 antigen and activity was measured in the conditioned medium and the cellular extracts after incubation of confluent cultures with 1% serum medium for 24 hours. Insulin induced a dose dependent increase of the PAI-1 secretion by Hep G2 cell line. At 10(-8) M a two fold increase of PAI-1 antigen and activity were observed whereas alpha 2 antiplasmin and fibrinogen were not significantly modified. No effect of insulin was observed on PAI-1 antigen and PAI activity production by human endothelial cells whereas endotoxin resulted in a two fold increase in PAI-1 secretion. In recent clinical studies we have demonstrated that the level of plasma insulin correlated with that of PAI-1. Thus we hypothesize that hepatocytes represent a physiological source of plasma PAI-1 which is modulated by plasma insulin level.
在胰岛素刺激后，对人脐静脉内皮细胞和人肝癌细胞系Hep G2的培养物中纤溶酶原激活物抑制剂1（PAI - 1）的分泌进行了评估。在用1%血清培养基培养汇合培养物24小时后，测量条件培养基和细胞提取物中PAI - 1抗原和活性的分泌。胰岛素诱导Hep G2细胞系分泌PAI - 1呈剂量依赖性增加。在10（-8）M时，观察到PAI - 1抗原和活性增加了两倍，而α2抗纤溶酶和纤维蛋白原没有明显改变。未观察到胰岛素对人内皮细胞产生的PAI - 1抗原和PAI活性有影响，而内毒素导致PAI - 1分泌增加两倍。在最近的临床研究中，我们已经证明血浆胰岛素水平与PAI - 1水平相关。因此，我们推测肝细胞是血浆PAI - 1的生理来源，其受血浆胰岛素水平调节。
