番茄（Lycopersicum esculentum）根际相关细菌的生物防治和促生能力的田间条件下。
Biocontrol and plant growth-promoting ability of plant-associated bacteria from tomato (Lycopersicum esculentum) under field condition.
机构信息
Department of Microbiology, Bharathidasan University, Tiruchirapalli, 620 024, Tamil Nadu, India; C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Bardoli, Surat, 394 350, Gujarat, India.
Crop Protection Division, Sugarcane Breeding Institute, Coimbatore, 641 007, Tamil Nadu, India.
出版信息
Microb Pathog. 2019 Nov;136:103713. doi: 10.1016/j.micpath.2019.103713. Epub 2019 Sep 3.
Forty-five bacterial isolates recovered from surface-sterilized root, stem and leaf tissues of tomato were studied for their antifungal activity against phytopathogens, and plant growth-promoting (PGP) and biocontrol traits. Six plant-associated bacteria suppressed all the pathogens tested under in vitro plate assay and also shown PGP and biocontrol traits. The six isolates showing PGP and biocontrol properties were identified as Bacillus spp., based on the microbial identification system (Biolog) and partial sequence analysis of 16S rDNA. Two independent field trials were conducted with biocontrol bacteria along with chemical control (Thiram+Fytolan) and control (Without treatment). The averaged results of two field trails revealed that tomato plants inoculated with BETS11 (11.73 t/ha) and BETR11 (11.24 t/ha) strains showed significantly higher yield and disease reduction on par with chemical control (11.81 t/ha). However, there was an increase in the yield with respect to uninoculated control except the isolate BETS5 (9.09 t/ha). Therefore, the isolates BETS11 and BETR11 may be used as efficient biofertilizer and bio-control agent for tomato production in the Island agricultural ecosystem.
从番茄表面消毒的根、茎和叶组织中分离出 45 株细菌，研究它们对植物病原菌的抗真菌活性，以及植物促生（PGP）和生物防治特性。在体外平板测定中，有 6 株植物相关细菌抑制了所有测试的病原体，同时还表现出 PGP 和生物防治特性。根据微生物鉴定系统（Biolog）和 16S rDNA 的部分序列分析，这 6 株具有 PGP 和生物防治特性的分离物被鉴定为芽孢杆菌属。进行了两项独立的田间试验，用生物防治细菌与化学防治（Thiram+Fytolan）和对照（未处理）一起进行。两项田间试验的平均结果表明，用 BETS11（11.73 吨/公顷）和 BETR11（11.24 吨/公顷）菌株接种的番茄植株的产量显著提高，与化学防治（11.81 吨/公顷）相当，且病害减少。然而，除了 BETS5 分离株（9.09 吨/公顷）外，与未接种对照相比，产量都有所增加。因此，BETS11 和 BETR11 分离株可作为有效的生物肥料和生物防治剂，用于岛屿农业生态系统中的番茄生产。
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