State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Int J Mol Sci. 2021 Nov 8;22(21):12065. doi: 10.3390/ijms222112065.
Plants serve as a niche for the growth and proliferation of a diversity of microorganisms. Soil microorganisms, which closely interact with plants, are increasingly being recognized as factors important to plant health. In this study, we explored the use of high-throughput DNA sequencing of the fungal ITS and bacterial 16S for characterization of the fungal and bacterial microbiomes following biocontrol treatment (DT) with strain Bv17 relative to treatments without biocontrol (DC) during the potato growth cycle at three time points. A total of 5631 operational taxonomic units (OTUs) were identified from the 16S data, and 2236 OTUs were identified from the ITS data. The number of bacterial and fungal OTU in DT was higher than in DC and gradually increased during potato growth. In addition, indices such as Ace, Chao, Shannon, and Simpson were higher in DT than in DC, indicating greater richness and community diversity in soil following the biocontrol treatment. Additionally, the potato tuber yields improved without a measurable change in the bacterial communities following the strain Bv17 treatment. These results suggest that soil microbial communities in the rhizosphere are differentially affected by the biocontrol treatment while improving potato yield, providing a strong basis for biocontrol utilization in crop production.
植物为多种微生物的生长和增殖提供了小生境。与植物密切相互作用的土壤微生物，越来越被认为是对植物健康很重要的因素。在这项研究中，我们探索了使用高通量 DNA 测序真菌 ITS 和细菌 16S，来描述在马铃薯生长周期中，与没有生物防治（DC）的处理相比，用 菌株 Bv17 进行生物防治（DT）后，真菌和细菌微生物组的特征。从 16S 数据中鉴定出了 5631 个操作分类单元（OTU），从 ITS 数据中鉴定出了 2236 个 OTU。DT 中的细菌和真菌 OTU 数量高于 DC，并且在马铃薯生长过程中逐渐增加。此外，在 DT 中，Ace、Chao、Shannon 和 Simpson 等指数高于 DC，表明生物防治处理后土壤中的丰富度和群落多样性更高。此外，在 菌株 Bv17 处理后，虽然细菌群落没有可测量的变化，但马铃薯块茎的产量提高了。这些结果表明，生物防治处理会对根际土壤微生物群落产生差异影响，同时提高马铃薯的产量，为生物防治在作物生产中的利用提供了坚实的基础。
