ICS-3 Soft Condensed Matter, Forschungszentrum Jülich GmbH, D-52428 Jülich, Germany.
J Phys Condens Matter. 2019 Dec 18;31(50):503003. doi: 10.1088/1361-648X/ab421c.
With rising popularity of microscale thermophoresis for the characterisation of protein-ligand binding reactions and possible applications in microfluidic devices, there is a growing interest in considering thermodiffusion in the context of life sciences. But although the understanding of thermodiffusion in non-polar mixtures has grown rapidly in recent years, predictions for associated mixtures like aqueous solutions remain challenging. This review aims to give an overview of the literature on thermodiffusion in aqueous systems, show the difficulties in theoretical description that arise from the non-ideal behaviour of water-mixtures, and highlight the relevance of thermodiffusion in a biological context. We find that the thermodiffusion in aqueous systems is dominated by contributions from heat of transfer, hydrogen bond interactions and charge effects. However, the separation of these effects is often difficult, especially in case of biological systems where a systematic exclusion of contributions may not be feasible.
随着微尺度热泳技术在蛋白质-配体结合反应的表征和微流控设备中的潜在应用的日益普及，人们对生命科学中热扩散的研究兴趣也在不断增加。但是，尽管近年来非极性混合物中热扩散的理解取得了快速进展，但对相关混合物（如水溶液）的预测仍然具有挑战性。本综述旨在概述有关水相系统中热扩散的文献，展示由于水混合物的非理想行为而导致理论描述所面临的困难，并强调在生物学背景下热扩散的相关性。我们发现，水相系统中的热扩散主要由热传递、氢键相互作用和电荷效应的贡献所主导。然而，这些效应的分离通常很困难，特别是在生物学系统中，系统地排除这些效应可能是不可行的。
