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比利时用于识别……的医院实验室调查。 (原文中“Identification of”后面似乎缺失了具体内容)

Hospital Laboratory Survey for Identification of in Belgium.

作者信息

Dewaele Klaas, Lagrou Katrien, Frans Johan, Hayette Marie-Pierre, Vernelen Kris

机构信息

Department of Clinical Microbiology, Imelda General Hospital, 2820 Bonheiden, Belgium.

Department of Laboratory Medicine and National Reference Centre for Mycosis, University Hospitals of Leuven, 3000 Leuven, Belgium.

出版信息

J Fungi (Basel). 2019 Sep 5;5(3):84. doi: 10.3390/jof5030084.

Abstract

is a difficult-to-identify, emerging yeast and a cause of sustained nosocomial outbreaks. Presently, not much data exist on laboratory preparedness in Europe. To assess the ability of laboratories in Belgium and Luxembourg to detect this species, a blinded strain was included in the regular proficiency testing rounds organized by the Belgian public health institute, Sciensano. Laboratories were asked to identify and report the isolate as they would in routine clinical practice, as if grown from a blood culture. Of 142 respondents, 82 (57.7%) obtained a correct identification of . Of 142 respondents, 27 (19.0%) identified the strain as . The remaining labs that did not obtain a correct identification (33/142, 23.2%), reported other yeast species (4/33) or failed to obtain a species identification (29/33). To assess awareness about the infection-control implications of the identification, participants were requested to indicate whether referral of this isolate to a reference laboratory was desirable in a clinical context. Over one-third of all respondents (54/142, 38.0%) stated that they would not send the isolate to a reference laboratory, neither for epidemiological reasons nor for identification confirmation or antifungal susceptibility testing. This comprised 41.5% of the laboratories that submitted an identification of (34/82). Awareness among Belgian microbiologists, therefore, remains inadequate more than two years after ' emergence in European clinics. Our data confirm high rates of misidentifications with commonly used identification methods. Programs for raising awareness in European hospitals may be warranted.

摘要

是一种难以识别的新兴酵母菌,也是持续性医院感染暴发的一个原因。目前,欧洲关于实验室应对准备的数据不多。为评估比利时和卢森堡的实验室检测该菌种的能力,在比利时公共卫生机构Sciensano组织的常规能力验证轮次中纳入了一株盲样菌株。要求实验室按照常规临床实践中对待从血培养中分离出的菌株那样,对该分离株进行鉴定并报告。在142名受访者中,82人(57.7%)对……做出了正确鉴定。在142名受访者中,27人(19.0%)将该菌株鉴定为……。其余未做出正确鉴定的实验室(33/142,23.2%)报告了其他酵母菌种(4/33)或未能鉴定出菌种(29/33)。为评估对该鉴定结果感染控制意义的知晓情况,要求参与者指出在临床情况下将此分离株送至参考实验室是否可取。超过三分之一的受访者(54/142,38.0%)表示,他们不会将该分离株送至参考实验室,既不是出于流行病学原因,也不是为了鉴定确认或抗真菌药敏试验。这占做出……鉴定的实验室的41.5%(34/82)。因此,在……在欧洲临床出现两年多后,比利时微生物学家的知晓情况仍然不足。我们的数据证实了常用鉴定方法的错误鉴定率很高。欧洲医院可能需要开展提高认识项目。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4f1/6787608/282b4b89dc12/jof-05-00084-g001.jpg

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