Carolus Hans, Jacobs Stef, Lobo Romero Celia, Deparis Quinten, Cuomo Christina A, Meis Jacques F, Van Dijck Patrick
Laboratory of Molecular Cell Biology, Department of Biology, Institute of Botany and Microbiology, KU Leuven, 3001 Leuven, Belgium.
VIB-KU Leuven Center for Microbiology, 3001 Leuven, Belgium.
J Fungi (Basel). 2021 Sep 13;7(9):754. doi: 10.3390/jof7090754.
is an opportunistic pathogenic yeast that emerged worldwide during the past decade. This fungal pathogen poses a significant public health threat due to common multidrug resistance (MDR), alarming hospital outbreaks, and frequent misidentification. Genomic analyses have identified five distinct clades that are linked to five geographic areas of origin and characterized by differences in several phenotypic traits such as virulence and drug resistance. Typing of strains and the identification of clades can be a powerful tool in molecular epidemiology and might be of clinical importance by estimating outbreak and MDR potential. As has caused global outbreaks, including in low-income countries, typing strains quickly and inexpensively is highly valuable. We report five allele-specific polymerase chain reaction (AS-PCR) assays for the identification of and each of the five described clades of based on conserved mutations in the internal transcribed spacer (ITS) rDNA region and a clade-specific gene cluster. This PCR method provides a fast, cheap, sequencing-free diagnostic tool for the identification of , clades, and potentially, the discovery of new clades.
是一种在过去十年中在全球范围内出现的机会致病性酵母。由于常见的多重耐药性（MDR）、令人担忧的医院暴发以及频繁的错误鉴定，这种真菌病原体对公共卫生构成了重大威胁。基因组分析已经确定了五个不同的进化枝，它们与五个起源地理区域相关联，并以毒力和耐药性等几个表型特征的差异为特征。菌株分型和进化枝鉴定在分子流行病学中可能是一种强大的工具，并且通过估计暴发和MDR潜力可能具有临床重要性。由于已导致全球暴发，包括在低收入国家，快速且廉价地对菌株进行分型非常有价值。我们报告了五种等位基因特异性聚合酶链反应（AS-PCR）检测方法，用于基于内部转录间隔区（ITS）rDNA区域的保守突变和一个进化枝特异性基因簇来鉴定以及所描述的五个进化枝中的每一个。这种PCR方法为鉴定、进化枝以及潜在地发现新进化枝提供了一种快速、廉价、无需测序的诊断工具。
