叶酸偶联氧化铁纳米粒子的铜放射性标记合成用于癌症诊断。

Synthesis of Cu-Radiolabeled Folate-Conjugated Iron Oxide Nanoparticles for Cancer Diagnosis.

机构信息

Department of Chemistry, Dongguk University, Jung-gu, Seoul-04620, Republic of Korea.

Materials Department, Biot Korea Inc., 43-26, Cheomdangwagi-ro, 208 Beon-gil, Buk-gu, Gwangju 61001, Republic of Korea.

出版信息

J Nanosci Nanotechnol. 2020 Apr 1;20(4):2040-2044. doi: 10.1166/jnn.2020.17205.

DOI:10.1166/jnn.2020.17205

PMID:31492210

Abstract

We report the synthesis of Cu-labeled folate-conjugated magnetic nanoparticles for positron emission tomography (PET)/magnetic resonance imaging (MRI)-based diagnosis of cancer. The citrate/hydrazine-stabilized Fe₃O₄ nanoparticles measuring ~50 nm were utilized for target synthesis. Radio-thin-layer chromatography (TLC) analysis shows the formation of Cu-labeled nanomaterial with a radiochemical purity of 82.17% and a stability of ~90% in buffer solution and human serum for 24 h. Moreover, the sample exhibited good cellular uptake in breast (SKBR3), oral (KB) and lung (A549) cancer cell lines.

摘要

我们报告了一种用于正电子发射断层扫描（PET）/磁共振成像（MRI）的基于癌症诊断的叶酸偶联磁性纳米粒子的铜标记合成。利用柠檬酸/肼稳定的 Fe₃O₄纳米粒子，其尺寸约为 50nm。放射性薄层层析（TLC）分析表明，在缓冲溶液和人血清中，形成了放射性化学纯度为 82.17%，稳定性约为 90%的铜标记纳米材料，24 小时后依然稳定。此外，该样品在乳腺癌（SKBR3）、口腔癌（KB）和肺癌（A549）癌细胞系中表现出良好的细胞摄取。