Laboratory for Cell Technology and Biotransformation, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000, Zagreb, Croatia.
Mol Biol Rep. 2019 Dec;46(6):6079-6085. doi: 10.1007/s11033-019-05043-8. Epub 2019 Sep 6.
Food proteins from different sources can provide beneficial effects on human health by releasing the bioactive peptides that are integral part of their native structure. In this study, we tested the biological potential of hempseed protein hydrolysates (HPHs) obtained from hempseed cake protein isolate. The HPHs were prepared by enzyme hydrolysis using three different proteases of microbial origin: Alcalase®, Neutrase® and Protamex®. The antioxidant activity of the obtained hydrolysates was determined by oxygen radical absorbance capacity (ORAC) assay, while the proliferative effects on normal (HaCaT) and cancer (HeLa) cells were determined by the CellTiter 96 AQ One Solution Reagent (MTS) assay. HPHs showed dose-dependent antiproliferative effects on HeLa cells and stimulatory effects on the proliferation of HaCaT cells. HPH obtained by Neutrase (HPH-N) showed the highest antioxidant activity expressed as an ORAC value. The protective effect of HPH-N on HO-induced oxidative stress in normal and cancer cells was evaluated and 1 mg/mL of HPH-N significantly reduced the formation of intracellular reactive oxygen species (ROS) in both cell lines. The obtained results indicate the benefits of HPHs as potential natural antioxidants for the food industry and contribute to the growing trend of utilizing hempseed by-products.
来自不同来源的食物蛋白质可以通过释放其天然结构中不可或缺的生物活性肽，对人体健康产生有益的影响。在这项研究中，我们测试了从大麻籽饼蛋白分离物中获得的大麻籽蛋白水解物（HPH）的生物学潜力。HPH 是通过使用三种不同的微生物来源的蛋白酶：Alcalase®、Neutrase®和Protamex®进行酶水解制备的。通过氧自由基吸收能力（ORAC）测定法测定所得水解物的抗氧化活性，而通过 CellTiter 96 AQ One Solution Reagent（MTS）测定法测定对正常（HaCaT）和癌细胞（HeLa）的增殖作用。HPH 对 HeLa 细胞表现出剂量依赖性的抗增殖作用，并对 HaCaT 细胞的增殖具有刺激作用。用 Neutrase 获得的 HPH（HPH-N）显示出最高的抗氧化活性，以 ORAC 值表示。评估了 HPH-N 对 HO 诱导的正常和癌细胞中氧化应激的保护作用，1mg/mL 的 HPH-N 可显著减少两种细胞系中细胞内活性氧物质（ROS）的形成。所得结果表明 HPH 作为食品工业中潜在的天然抗氧化剂的益处，并有助于利用大麻籽副产物的增长趋势。
