Instituto de Farmacobiología, Universidad de la Cañada, Carretera Teotitlán - San Antonio Nanahuatipán Km 1.7 s/n., Paraje Titlacuatitla, Teotitlán de Flores Magón, Oaxaca, 68540, México.
Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México, Instituto Tecnológico de Tepic, Tepic - Avenida Tecnológico #2595, Colonia Lagos del Country, Tepic, Nayarit, 63175, México.
Plant Foods Hum Nutr. 2024 Sep;79(3):685-692. doi: 10.1007/s11130-024-01203-9. Epub 2024 Jul 10.
Jackfruit leaf protein hydrolysates obtained from the enzymatic hydrolysis of leaf protein concentrate with gastrointestinal enzymes have shown good techno-functional properties and high antioxidant capacity. However, molecular weight, antiproliferative activity, cytotoxicity and the ability to reduce reactive oxygen species (ROS) are still unknown. Therefore, this study aimed to evaluate the effect of jackfruit leaf protein hydrolysates obtained by enzymatic hydrolysis with pepsin and pancreatin at different hydrolysis times (30-240 min) on molecular weights, cytotoxicity, antiproliferation of cancer cells, and the reduction of reactive oxygen species in HO-induced HaCaT cells. The electrophoretic profile indicated that H-Pep contains peptides with molecular weights between 25 - 20 kDa. Meanwhile, H-Pan is composed of molecular weight products between 25 - 20 kDa and < 20 kDa. H-Pan and H-Pep (125-500 µg/mL) did not show significant cytotoxicity on HaCaT (human keratinocytes) and J774A.1 (murine macrophage cells). Antiproliferative activity was achieved in human cervical, ovarian, and liver cancer cells. H-Pan-240 min (1000 µg/mL) reduced the cell viability of cervical cancer cells by 23% while H-Pan-60 min significantly reduced cell viability of ovarian and liver cancer cells by 14.5 (500 µg/mL) and 17% (1000 µg/mL), respectively (P < 0.05). The protective effect against oxidative stress on HO-stressed HaCaT cells was obtained with H-Pep-60 min, which reduced 25% of ROS at 250 µg/mL (P < 0.05). The findings demonstrate the safe use of green biomass as a source of plant protein hydrolysates.
菠萝蜜叶蛋白水解物通过胃蛋白酶和胰蛋白酶对叶蛋白浓缩物进行酶解获得，具有良好的技术功能特性和高抗氧化能力。然而，其分子量、抗增殖活性、细胞毒性以及降低活性氧（ROS）的能力仍不清楚。因此，本研究旨在评估不同水解时间（30-240 分钟）下用胃蛋白酶和胰蛋白酶酶解菠萝蜜叶蛋白获得的水解物对分子质量、细胞毒性、癌细胞增殖抑制和 HO 诱导的 HaCaT 细胞中活性氧减少的影响。电泳图谱表明，H-Pep 含有分子量在 25-20 kDa 之间的肽。同时，H-Pan 由分子量在 25-20 kDa 和 <20 kDa 之间的产物组成。H-Pan 和 H-Pep（125-500 μg/mL）对 HaCaT（人角质形成细胞）和 J774A.1（鼠巨噬细胞）没有明显的细胞毒性。对人宫颈癌、卵巢癌和肝癌细胞具有抗增殖活性。H-Pan-240 min（1000 μg/mL）使宫颈癌细胞活力降低 23%，而 H-Pan-60 min 使卵巢癌和肝癌细胞活力分别显著降低 14.5%（500 μg/mL）和 17%（1000 μg/mL）（P<0.05）。H-Pep-60 min 对 HO 应激的 HaCaT 细胞具有抗氧化应激的保护作用，在 250 μg/mL 时降低了 25%的 ROS（P<0.05）。研究结果表明，绿色生物量作为植物蛋白水解物的来源可以安全使用。
