在沉降速度中使用三通道中心部件进行高效数据采集。
Efficient data acquisition with three-channel centerpieces in sedimentation velocity.
机构信息
Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA; Biophysical Immunology Laboratory, Department of Biomedicine, Aarhus University, Aarhus, Denmark.
Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
出版信息
Anal Biochem. 2019 Dec 1;586:113414. doi: 10.1016/j.ab.2019.113414. Epub 2019 Sep 4.
Three-channel 3D printed centerpieces with two sample sectors next to a joint solvent reference sector were recently described as a strategy to double the throughput of sedimentation velocity analytical ultracentrifugation experiments [Anal. Chem. 91 (2019) 5866-5873]. They are compatible with Rayleigh interference optical detection in commercial analytical ultracentrifuges, but require the rotor angles of data acquisition to be repeatedly adjusted during the experiment to record data from the two sample sectors. Here we present an approach to automate this data acquisition mode through the use of a secondary, general-purpose automation software, and an accompanying data pre-processing software for scan sorting.
最近有人描述了一种三通道 3D 打印的中心件，其旁边有两个样品扇区和一个联合溶剂参照扇区，这是一种将沉降速度分析超速离心实验通量提高一倍的策略[Anal. Chem. 91 (2019) 5866-5873]。这些中心件与商业分析超速离心机中的瑞利干涉光学检测兼容，但需要在实验过程中反复调整数据采集转子角度，以记录来自两个样品扇区的数据。在这里，我们通过使用辅助的通用自动化软件和配套的扫描分类数据预处理软件，提出了一种自动化此数据采集模式的方法。
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