牛卵巢细胞中转录因子 E2F8 基因表达的调控。

Regulation of the transcription factor E2F8 gene expression in bovine ovarian cells.

机构信息

Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK, 74078, USA.

出版信息

Mol Cell Endocrinol. 2019 Dec 1;498:110572. doi: 10.1016/j.mce.2019.110572. Epub 2019 Sep 4.

DOI:10.1016/j.mce.2019.110572

Abstract

Overexpression of the transcription factor, E2F8, has been associated with ovarian cancer. Objectives of this study were to determine: 1) if E2F8 gene expression in granulosa cells (GC) and theca cells (TC) change with follicular development, and 2) if E2F8 mRNA abundance in TC and GC is hormonally regulated. Using real-time PCR, E2F8 mRNA abundance in GC and TC was greater (P < 0.05) in small than large follicles. FGF9 induced an increase (P < 0.05) in E2F8 mRNA abundance by 1.6- to 7-fold in large-follicle (8-20 mm) TC and GC as well as in small-follicle (1-5 mm) GC. Abundance of E2F8 mRNA in TC was increased (P < 0.05) with FGF2, FGF9 or VEGFA treatments alone in vitro, and concomitant treatment of VEGFA with FGF9 increased (P < 0.05) abundance of E2F8 mRNA above any of the singular treatments; BMP4, WNT3A and LH were without effect. IGF1 amplified the stimulatory effect of FGF9 on E2F8 mRNA abundance by 2.7-fold. Collectively, our studies show for the first time that follicular E2F8 is developmentally and hormonally regulated indicating that E2F8 may be involved in follicular development.

摘要

转录因子 E2F8 的过表达与卵巢癌有关。本研究的目的是确定：1）E2F8 在颗粒细胞 (GC) 和膜细胞 (TC) 中的基因表达是否随卵泡发育而变化，以及 2）TC 和 GC 中的 E2F8 mRNA 丰度是否受激素调节。使用实时 PCR，GC 和 TC 中的 E2F8 mRNA 丰度在小卵泡（<10mm）中高于大卵泡（>10mm）（P<0.05）。FGF9 诱导大卵泡（8-20mm）TC 和 GC 以及小卵泡（1-5mm）GC 中 E2F8 mRNA 丰度增加（P<0.05），增加 1.6-7 倍。FGF2、FGF9 或 VEGFA 单独处理时 TC 中 E2F8 mRNA 的丰度增加（P<0.05），VEGFA 与 FGF9 同时处理时 E2F8 mRNA 的丰度增加（P<0.05）高于任何单独处理；BMP4、WNT3A 和 LH 没有影响。IGF1 将 FGF9 对 E2F8 mRNA 丰度的刺激作用放大了 2.7 倍。总之，我们的研究首次表明，卵泡 E2F8 受发育和激素调节，表明 E2F8 可能参与卵泡发育。