Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia; Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", 50/50 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia.
Int J Biol Macromol. 2019 Dec 1;141:765-775. doi: 10.1016/j.ijbiomac.2019.09.015. Epub 2019 Sep 4.
Four PHA types were synthesized in the culture of Cupriavidus eutrophus B-10646 under special conditions, poly(3‑hydroxybutyrate) [P(3HB)] and of copolymers, which contained 3HB monomers and 4‑hydroxybutyrate (4HB), 3‑hydroxyvalerate (3HV), or 3‑hydroxyhexanoate (3HHx). All copolymers had the M of about 550-670 kDa, and the homopolymer P(3HB) had a significantly higher M - 920 kDa. P(3HB‑co‑4HB) and P(3HB‑co‑3HHx) had the lowest C (42 and 49%) while P(3HB‑co‑3HV) and P(3HB) exhibited higher C values (76%). Polymer films were prepared from different PHAs. Electron microscopy showed differences in the surface microstructure of the films. Films prepared from the P(3HB) were more hydrophobic and the arithmetic mean surface roughness of 71-75 nm, than the copolymer films, which were hydrophilic (57-60°) and had considerably higher roughness (158-177 nm). Blood parameters (hemoglobin and hemolysis) and response of the cells (erythrocytes, platelets, and monocytes) were studied in experiments with blood directly contacting the surface of the films of PHAs with different compositions. Cultivation of blood cells on polymer films did not cause any adverse effects on adhesion and morphology of all cell types. Results of studying blood cell response suggested that the films made from low-crystallinity copolymers containing 4‑hydroxybutyrate and 3‑hydroxyhexanoate were the best for contact with blood.
在特殊条件下，铜绿假单胞菌 B-10646 的培养物中合成了四种 PHA 类型，包括聚 3-羟基丁酸酯（P(3HB)）和共聚物，共聚物中含有 3HB 单体和 4-羟基丁酸酯（4HB）、3-羟基戊酸酯（3HV）或 3-羟基己酸酯（3HHx）。所有共聚物的 M 约为 550-670 kDa，而均聚物 P(3HB)的 M 显著更高-920 kDa。P(3HB-co-4HB)和 P(3HB-co-3HHx)的 C 值最低（42%和 49%），而 P(3HB-co-3HV)和 P(3HB)的 C 值较高（76%）。不同的 PHAs 制备了聚合物薄膜。电子显微镜显示了薄膜表面微观结构的差异。由 P(3HB)制备的薄膜疏水性更强，算术平均表面粗糙度为 71-75 nm，而共聚物薄膜亲水性更强（57-60°），表面粗糙度更高（158-177 nm）。直接接触不同组成的 PHAs 薄膜表面的血液参数（血红蛋白和溶血）和细胞（红细胞、血小板和单核细胞）反应的实验中，研究了血液参数和细胞反应。在聚合物薄膜上培养血细胞不会对所有细胞类型的粘附和形态产生任何不良影响。血细胞反应研究结果表明，含有 4-羟基丁酸酯和 3-羟基己酸酯的低结晶度共聚物制成的薄膜与血液接触最佳。
