Shishatskaya Ekaterina I, Dudaev Alexey E, Volova Tatiana G
Department of Medical Biology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, Russia.
Chemistry Engineering Centre, ITMO University, Kronverkskiy Prospekt, 49A, 197101 Saint Petersburg, Russia.
Nanomaterials (Basel). 2022 Oct 31;12(21):3843. doi: 10.3390/nano12213843.
From a series of biodegradable natural polymers of polyhydroxyalkanoates (PHAs)-poly-3-hydroxybutyrate (P(3HB) and copolymers containing, in addition to 3HB monomers, monomers of 3-hydroxyvalerate (3HV), 3-hydroxyhexanoate (3HHx), and 4-hydroxybutyrate (4HB), with different ratios of monomers poured-solvent casting films and nanomembranes with oriented and non-oriented ultrathin fibers were obtained by electrostatic molding. With the use of SEM, AFM, and measurement of contact angles and energy characteristics, the surface properties and mechanical and biological properties of the polymer products were studied depending on the method of production and the composition of PHAs. It has been shown in cultures of mouse fibroblasts of the NIH 3T3 line and diploid human embryonic cells of the M22 line that elastic films and nanomembranes composed of P(3HB-co-4HB) copolymers have high biocompatibility and provide adhesion, proliferation and preservation of the high physiological activity of cells for up to 7 days. Polymer films, namely oriented and non-oriented nanomembranes coated with type 1 collagen, are positively evaluated as experimental wound dressings in experiments on laboratory animals with model and surgical skin lesions. The results of planimetric measurements of the dynamics of wound healing and analysis of histological sections showed the regeneration of model skin defects in groups of animals using experimental wound dressings from P(3HB-co-4HB) of all types, but most actively when using non-oriented nanomembranes obtained by electrospinning. The study highlights the importance of nonwoven nanomembranes obtained by electrospinning from degradable low-crystalline copolymers P(3HB-co-4HB) in the effectiveness of the skin wound healing process.
从一系列聚羟基脂肪酸酯（PHA）的可生物降解天然聚合物——聚-3-羟基丁酸酯（P(3HB)）以及除3HB单体外还含有3-羟基戊酸酯（3HV）、3-羟基己酸酯（3HHx）和4-羟基丁酸酯（4HB）单体的共聚物中，通过静电成型获得了具有不同单体比例的浇铸膜和具有取向和非取向超细纤维的纳米膜。利用扫描电子显微镜（SEM）、原子力显微镜（AFM）以及接触角和能量特性的测量，根据生产方法和PHA的组成研究了聚合物产品的表面性能、机械性能和生物学性能。在NIH 3T3小鼠成纤维细胞培养物和M22二倍体人胚胎细胞培养物中已表明，由P(3HB-co-4HB)共聚物组成的弹性膜和纳米膜具有高生物相容性，并能在长达7天的时间内提供细胞的粘附、增殖和高生理活性的维持。聚合物膜，即涂有I型胶原蛋白的取向和非取向纳米膜，在患有模型和手术性皮肤损伤的实验动物实验中作为实验性伤口敷料得到了积极评价。伤口愈合动力学的平面测量结果和组织学切片分析表明，在使用所有类型的P(3HB-co-4HB)实验性伤口敷料的动物组中，模型皮肤缺损均有再生，但在使用通过静电纺丝获得的非取向纳米膜时最为活跃。该研究突出了通过静电纺丝从可降解低结晶共聚物P(3HB-co-4HB)获得的非织造纳米膜在皮肤伤口愈合过程有效性中的重要性。
