Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Obstetrics and Gynecology, Kaohsiung Municipal Siaogang Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
Biomed Pharmacother. 2019 Nov;119:109105. doi: 10.1016/j.biopha.2019.109105. Epub 2019 Sep 4.
Endometriosis is considered a high risk factor for the development of ovarian carcinoma, including clear cell and endometrioid malignancies. The mechanism by which endometriosis-associated ovarian cancer (EAOC) avoids anti-tumor immune surveillance by macrophages remains unclear, but CD47 is a very important immune checkpoint for macrophage phagocytosis. Therefore, we collected 36 clinical ovarian samples and detected the protein profile of CD47 by immunohistochemistry and analyzed the correlation with clinical pathological features using statistical software. We found that CD47 expression was relatively higher in patients with EAOC compared with the normal group. High CD47 expression was positively and significantly correlated with histology (P = 0.007) and tumor grade (P = 0.002). We also found that CD47 overexpression promotes cancer cell growth and motility in the TOV-112D and TOV-21G cell lines. Silencing CD47 and anti-CD47 mAb inhibit cancer cell growth and motility in cancer cell lines. Together, these results demonstrate that CD47 in EAOC may be a useful surface marker and offer a novel therapeutic option by targeting CD47 in ovarian cancer.
子宫内膜异位症被认为是卵巢癌发展的一个高危因素，包括透明细胞癌和子宫内膜样癌。子宫内膜异位症相关卵巢癌（EAOC）如何逃避巨噬细胞的抗肿瘤免疫监视尚不清楚，但 CD47 是巨噬细胞吞噬作用的一个非常重要的免疫检查点。因此，我们收集了 36 例临床卵巢样本，通过免疫组织化学检测 CD47 的蛋白谱，并使用统计软件分析与临床病理特征的相关性。我们发现 EAOC 患者的 CD47 表达相对高于正常组。高 CD47 表达与组织学（P=0.007）和肿瘤分级（P=0.002）呈正显著相关。我们还发现 CD47 过表达促进 TOV-112D 和 TOV-21G 细胞系中癌细胞的生长和运动。沉默 CD47 和抗 CD47 mAb 抑制癌细胞系中癌细胞的生长和运动。综上所述，这些结果表明 EAOC 中的 CD47 可能是一个有用的表面标志物，并通过靶向卵巢癌中的 CD47 提供了一种新的治疗选择。
