Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland; FutureSynthesis Sp. z o.o., Rubież 46H, 61-612 Poznań, Poland.
FutureSynthesis Sp. z o.o., Rubież 46H, 61-612 Poznań, Poland.
Bioorg Chem. 2019 Nov;92:103223. doi: 10.1016/j.bioorg.2019.103223. Epub 2019 Aug 28.
This 2-part article reviews methods of oligonucleotides functionalization with thiol tethers and their consecutive use in conjugation with other (macro)molecules via a disulfide bridge. This relatively inexpensive, robust and reversible method of conjugation of DNAs, RNAs and their analogs holds a prominent position in a modern biochemistry toolbox and therefore there is a wealth of literature on the subject. In part I methods of thiol/disulfide groups introduction into oligonucleotide strands have been systematized and discussed. A digest of conjugation methods is presented as well.
这篇由两部分组成的文章回顾了带有硫醇连接物的寡核苷酸功能化的方法，以及随后通过二硫键与其他（大分子）分子进行连接的方法。这种相对廉价、稳健且可还原的 DNA、RNA 及其类似物的连接方法在现代生物化学工具包中占据着重要地位，因此，关于这个主题的文献很多。在第一部分中，我们对寡核苷酸链中引入巯基/二硫键的方法进行了系统的总结和讨论。同时也介绍了连接方法的摘要。
