IEEE Trans Biomed Eng. 2020 Jun;67(6):1542-1547. doi: 10.1109/TBME.2019.2939560. Epub 2019 Sep 5.
This article presents a localized surface plasmon resonance (LSPR) phenomenon based optical fiber sensor (OFS) for the detection of dopamine (DA). DA functions as a hormone and a neurotransmitter in the human body and plays a crucial role in the peripheral system. To develop the OFS for DA detection, taper fiber probe was fabricated and immobilized with silver nanoparticles (AgNPs) and functionalized with Polyethylene glycol (PEG). The developed sensor shows the great selectivity in the presence of ascorbic acid (AA) oxidation due to PEG coating. The morphology of the AgNPs and uniformity of coating over the surface of sensing probe were confirmed with UV-visible spectrophotometer, transmission electron microscope (TEM), energy-dispersive X-ray spectroscopy (EDS), and scanning electron microscope (SEM). The calibration curve is found to be linear over the range of 10 nM-1 μM with the lowest detection limit of 0.058 μM. Also provides a wide dynamic range of detection (10 nm-100 μM). The parameters responsible for the performance of OFS, such as sensitivity, detection limit, and selectivity are greatly improved in the proposed sensor. The applicability of the proposed sensor has been validated and have the potential to use for routine diagnosis.
本文提出了一种基于局域表面等离子体共振（LSPR）现象的光纤传感器（OFS），用于检测多巴胺（DA）。DA 作为人体中的激素和神经递质，在周围系统中起着至关重要的作用。为了开发用于 DA 检测的 OFS，我们制备了锥形光纤探针，并将其固定在银纳米粒子（AgNPs）上，并进行了聚乙二醇（PEG）功能化。由于 PEG 涂层的存在，开发的传感器在抗坏血酸（AA）氧化存在下表现出了很好的选择性。AgNPs 的形态和表面涂层的均匀性通过紫外-可见分光光度计、透射电子显微镜（TEM）、能量色散 X 射线光谱（EDS）和扫描电子显微镜（SEM）进行了确认。发现校准曲线在 10 nM-1 μM 的范围内呈线性，最低检测限为 0.058 μM。还提供了 10nm-100 μM 的宽检测动态范围。在提出的传感器中，大大提高了 OFS 的性能参数，如灵敏度、检测限和选择性。已经验证了提出的传感器的适用性，并具有用于常规诊断的潜力。
